Home Resources Video Library What are the best conditions for DNA ligation?

What are the best conditions for DNA ligation?

Find out how the downstream application dictates the best reaction conditions for ligation.

Script

Eric Cantor:

Optimal reaction conditions depend on the downstream step. For library construction, low temperatures such as 16 degrees Celsius and long incubation times, 12-16 hours, provide the best reaction yields. This allows you to maximize library complexity. Complexity is not important for subcloning of a single insert into a vector; therefore, you can accomplish this with a much shorter reaction time, 15 minutes or less, over a much broader range of temperatures, 16 degrees Celsius to 37 degrees Celsius.

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