New England Biolabs: Reagents for the Life Sciences Industry

DNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.


Blunt/TA
Ligase
Master Mix
Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
Ligation™
Kit
T3 DNA
Ligase
T7 DNA
Ligase
HiFi Taq DNA Ligase
Taq DNA
Ligase
9°N™ DNA
Ligase
NEBNext Quick Ligation™ Module SplintR® Ligase  E.coli DNA Ligase
DNA APPLICATIONS
Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★ ★ 
   ★
Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★
 


   
T/A cloning ★★★ ★★ ★★ ★★  


   
Electroporation

★★★ ★★


 


   
Ligation of sticky ends only





★★★  


   
Repair of nicks in dsDNA ★★ ★★ ★★ ★★★ ★★ ★★ ★★ ★★  ★★ ★★
★★ ★★ 
High-complexity library cloning ★★ ★★ ★★ ★★★ ★★

 


   
Adapter Ligation  ▲
★★★ ★★ ★★ ★★
 

   
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)






 ★★★ ★★ ★★
   
Ligation-Dependent RNA Sequence & SNP Detection





 


★★★  
Ligation of adjacent ssDNAs on an RNA splint






     
 ★★★  
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)



 

   
FEATURES
Salt tolerance
(>2X that for T4 DNA Ligase)






 


   
Ligation in 15 min. or less

 ✓  
Master Mix Formulation




 

   
Thermostable






 ✓
   
Recombinant  ✓
KEY
★★★ Optimal, recommended ligase for selected application
★★ Works well for selected application
Will perform selected application, but is not recommended
Please consult the specific NGS protocol to determine the optimal enzyme for your needs

ElectroLigase™, SplintR®, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.