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  • DNA Ligase Selection Chart

    While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.

    Blunt/TA
    Ligase
    Master Mix
    Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
    Ligation™
    Kit
    T3 DNA
    Ligase
    T7 DNA
    Ligase
    E. coli DNA
    Ligase
    Taq DNA
    Ligase
    9°N™ DNA
    Ligase
    Quick Ligation™ Module
    DNA APPLICATIONS
    Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★
    Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★
    T/A cloning ★★★ ★★ ★★ ★★
    Electroporation ★★★ ★★
    Ligation of sticky ends only ★★★
    Repair of nicks in dsDNA ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★
    High-complexity library cloning ★★ ★★ ★★ ★★ ★★
    Ligase Detection Reaction & Ligase Chain Reaction ★★ ★★★
    DNA/RNA APPLICATIONS
    Joining of RNA & DNA in a ds-structure ★★★ ★★★
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)
    FEATURES
    Salt tolerance
    (>2X that for T4 DNA Ligase)
    Ligation in 15 min. or less
    Master Mix Formulation
    Thermostable
    Recombinant
    KEY
    ★★★ Optimal, recommended ligase for selected application
    ★★ Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs

    ElectroLigase™, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.