DNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart.  In addition, the Properties of DNA and RNA Ligases will help you understand the differences between them.


Blunt/TA
Ligase
Master Mix
Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Hi-T4™ DNA Ligase  Salt-T4® DNA Ligase  Quick
Ligation™
Kit
T3 DNA
Ligase
T7 DNA
Ligase
HiFi Taq DNA Ligase
Taq DNA
Ligase
9°N™ DNA
Ligase
NEBNext Quick Ligation™ Module SplintR® Ligase  E.coli DNA Ligase
DNA APPLICATIONS
Ligation of sticky ends ★★ ★★★ ★★ ★★  ★★ ★★
★★★ ★★ ★★ ★ 
   ★
Ligation of blunt ends ★★★ ★★ ★★  ★★ ★★
★★★ ★★
 


   
T/A cloning ★★★ ★★ ★★  ★★ ★★
★★  


   
Electroporation

★★★ ★★  ★★



 


   
Ligation of sticky ends only



   

★★★  


   
Repair of nicks in dsDNA ★★ ★★ ★★ ★★★  ★★★ ★★★
★★ ★★ ★★ ★★  ★★ ★★
★★ ★★ 
High-complexity library cloning ★★ ★★ ★★ ★★★     ★★

 


   
Adapter Ligation  ▲
★★★ ★★ ★★     ★★
 

   
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)



   


 ★★★ ★★ ★★
   
Ligation-Dependent RNA Sequence & SNP Detection


   


 


★★★  
Ligation of adjacent ssDNAs on an RNA splint



   


     
 ★★★  
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)

   

 

   
FEATURES
Salt tolerance
(>2X that for T4 DNA Ligase)




   

 


   
Ligation in 15 min. or less
   
 ✓  
Master Mix Formulation

   


 

   
Thermostable



   


 ✓
   
Thermotolerant



 


 ✓
   
Recombinant  ✓     ✓
KEY
★★★ Optimal, recommended ligase for selected application
★★ Works well for selected application
Will perform selected application, but is not recommended
Please consult the specific NGS protocol to determine the optimal enzyme for your needs
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