DNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.


Blunt/TA
Ligase
Master Mix
Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
Ligation™
Kit
T3 DNA
Ligase
T7 DNA
Ligase
HiFi Taq DNA Ligase
Taq DNA
Ligase
9°N™ DNA
Ligase
NEBNext Quick Ligation™ Module SplintR® Ligase  E.coli DNA Ligase
DNA APPLICATIONS
Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★ ★ 
   ★
Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★
 


   
T/A cloning ★★★ ★★ ★★ ★★  


   
Electroporation

★★★ ★★


 


   
Ligation of sticky ends only





★★★  


   
Repair of nicks in dsDNA ★★ ★★ ★★ ★★★ ★★ ★★ ★★ ★★  ★★ ★★
★★ ★★ 
High-complexity library cloning ★★ ★★ ★★ ★★★ ★★

 


   
Adapter Ligation  ▲
★★★ ★★ ★★ ★★
 

   
Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)






 ★★★ ★★ ★★
   
Ligation-Dependent RNA Sequence & SNP Detection





 


★★★  
Ligation of adjacent ssDNAs on an RNA splint






     
 ★★★  
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)



 

   
FEATURES
Salt tolerance
(>2X that for T4 DNA Ligase)






 


   
Ligation in 15 min. or less

 ✓  
Master Mix Formulation




 

   
Thermostable






 ✓
   
Recombinant  ✓
KEY
★★★ Optimal, recommended ligase for selected application
★★ Works well for selected application
Will perform selected application, but is not recommended
Please consult the specific NGS protocol to determine the optimal enzyme for your needs

ElectroLigase™, SplintR®, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.

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