For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
50X higher fidelity than Taq
Robust reactions - maximal success with minimal optimization
Offered with multiple buffers for customized reaction setup for different DNA templates
Product Information
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl2 solutions.
Figure 1: Fidelity of DNA polymerases Relative to Taq
Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
Figure 2: Phusion DNA Polymerase generates robust amplification even with short extension times
A 1.2 kb C. elegans genomic amplicon was analyzed using different polymerases. Reactions were set up in duplicate and visualized by microfluidic LabChip analysis. All reactions were conducted according to manufacturer’s instructions using 30 cycles and extension times of either 30 seconds or 2 minutes as indicated.
Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.
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Phusion has different annealing temperature requirements than most PCR enzymes. Please check out NEB's Tm Calculator to help you determine your optimal Phusion annealing temperature.
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Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Phusion™ DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.
PHUSION™ and THERMO SCIENTIFIC™ are trademarks and property of Thermo Fisher Scientific.
Notice to purchaser:
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.
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