Q5® High-Fidelity DNA Polymerases
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.
Find the right Q5 product for your application
The five features of Q5 High-Fidelity DNA Polymerase
- Extremely low error rates
At ~280X higher than Taq, Q5 offers unparalleled fidelity for your most important samples.
- Robust amplification with minimal optimization
High specificity and yield are absolute requirements for today's molecular biology techniques.
- Superior coverage for a broad range of amplicons, regardless of GC content
While other DNA polymerases can have difficulty amplifying high-GC or high-AT amplicons, Q5 displays superior performance for a wide range of templates.
Robust Amplification with Q5 (A) and Q5 Hot Start (B) High-Fidelity DNA Polymerases
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Shorter PCR protocols
Achieve precision without sacrificing speed. Q5's unique design incorporating the SSo7d processivity-enhancing domain enables shorter extension times, as low as 10 seconds per kb. Additionally, aptamer-based hot start requires no initial denaturation step and enables room temperature setup.
- Amplify templates up to 20 kb
With Q5, you can reliably amplify simple templates up to 20 kb. Complex templates up to 10 kb can also be amplified with a high degree of confidence.
Master Mix and Stand-Alone Formats Provide Convenience and Flexibility
Q5® is a registered trademark of New England Biolabs, Inc.
LabChip® is a registered trademark of Caliper Life Sciences, part of Perkin Elmer, Inc.
Choose Product:
Choose Type:
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (M0541)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Protocol for Q5® High-Fidelity 2X Master Mix
- PCR Optimization (E0555)
- Protocol for a Routine PCR (E0555)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
- Protocol for a PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (M0544)
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
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Polymerase Fidelity: What is it, and what does it mean for your PCR?
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Using aptamers to control enzyme activity: Hot Start Taq and beyond
This article describes the use of engineered oligonucleotides known as aptamers in regulating the enzyme activity of polymerases and reverse transcriptases.
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Understanding Variability in DNA Amplification Reactions
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Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Reagents Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
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