Thermophilic DNA Polymerases
NEB supplies many thermophilic DNA polymerases; several characteristics should be considered when choosing the one best suited to your particular research needs.
|
Vent® |
Vent® (exo-) |
Deep Vent® |
Deep Vent® (exo-) |
Taq |
9°N™ |
Phusion®** |
Long Amp® Taq |
Long Amp® Hot Start Taq |
One Taq® |
Q5™ |
| Exonuclease Activities |
| proofreading exonuclease |
yes |
no |
yes |
no |
no |
no* |
yes |
yes |
yes |
yes |
yes |
| 5'—>3' exonuclease activity |
no |
no |
no |
no |
yes |
no |
no |
yes |
yes |
yes |
no |
| Thermal Stability |
| Half life at 95°C |
6.7 hours |
6.7 hours |
23 hours |
23 hours |
0.9 hours |
6.7 hours |
>6 hours at 96°C |
ND |
ND |
ND |
ND |
| Half life at 100°C |
1.8 hours |
1.8 hours |
8 hours |
8 hours |
<0.1 hours |
<1.8 hours |
2 hours at 98°C |
ND |
ND |
ND |
ND |
| Primer Extension Characteristics |
| Units/100 µl Primer Extension Rxn |
0.4-2 units |
1-4units |
0.4-2 units |
1-4 units |
2.5-5 units |
1-2 units |
1-2 units |
2-10 units |
2-10 units |
2.5-5 units |
1-2 units |
| Fidelity to Taq |
5X |
2X |
6X |
2X |
1X |
ND |
>50X |
2X |
2X |
2X |
>100X |
| Resulting DNA Ends |
blunt |
3´ A |
blunt |
3´ A |
3´ A |
3´ A blunt |
blunt |
3´ A
blunt |
3´ A
blunt |
3´ A
blunt |
blunt |
| Strand Displacement |
yes; temp. dependent |
yes; temp. dependent |
yes; temp. dependent |
yes; temp. dependent |
no |
yes |
no |
yes; temp. dependent |
yes; temp. dependent |
yes; temp. dependent |
no |
| Longest Primer Extension to Date |
13.2 kb |
15.0 kb |
14.0 kb |
15.0 kb |
8.1 kb |
37 kb |
40 kb |
40 kb |
15.0 kb |
20 kb |
20 kb |
Vent®, LongAmp® and OneTaq® are registered trademarks of New England Biolabs, Inc.
Deep Vent®, 9°N™ and Q5™ are trademarks of New England Biolabs, Inc.
* 1-5% of wild type exonuclease activity is present in the modified enzyme.
** Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. Under agreement with, and under the performance specifications of Thermo Fisher Scientific.
