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Product Information
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Restriction Enzyme Competitor Study: Nuclease Contamination
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing oligonucleotides. The percent degradation is determined by capillary electrophoresis and peak analysis. The resolution is at the single nucleotide level.Product Source
An E. coli strain that carries the cloned and modified SbfI gene from Streptomycesspecies Bf-61 (S.K. Degtyarev).- This product is related to the following categories:
- Restriction Endonucleases S Products,
- High-Fidelity (HF®) Restriction Endonucleases Products,
- Time-Saver Qualified Restriction Enzymes Products
- This product can be used in the following applications:
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
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Protocols, Manuals & Usage
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FAQs & Troubleshooting
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Citations & Technical Literature
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Additional Citations
- Hou Y, Nowak MD, Mirré V, Bjorå CS, Brochmann C, Popp M. (2016) RAD-seq data point to a northern origin of the arctic-alpine genus Cassiope (Ericaceae). Mol Phylogenet Evol; Feb;95, PubMedID: 152-60
- Kanamori A, Sugita Y, Yuasa Y, Suzuki T, Kawamura K, Uno Y, Kamimura K, Matsuda Y, Wilson CA, Amores A, Postlethwait JH, Suga K, Sakakura Y. (2016) A Genetic Map for the Only Self-Fertilizing Vertebrate. G3 (Bethesda); Apr 7;6(4), 1095-106.
- Kemppainen P, Knight CG, Sarma DK, Hlaing T, Prakash A, Maung Maung YN, Somboon P, Mahanta J, Walton C. (2015) Linkage disequilibrium network analysis (LDna) gives a global view of chromosomal inversions, local adaptation and geographic structure. Mol Ecol Resour; Sep;15(5), 1031-45.
- Demos TC, Kerbis Peterhans JC, Joseph TA, Robinson JD, Agwanda B, Hickerson MJ. (2015) Comparative Population Genomics of African Montane Forest Mammals Support Population Persistence across a Climatic Gradient and Quaternary Climatic Cycles. PLoS One; Sep 22;10(9), e0131800.
- Lescak EA, Bassham SL, Catchen J, Gelmond O, Sherbick ML, von Hippel FA, Cresko WA. (2015) Evolution of stickleback in 50 years on earthquake-uplifted islands. Proc Natl Acad Sci U S A; Dec 29;112(52), E7204-12.
- Herrera S, Watanabe H, Shank TM. (2015) Evolutionary and biogeographical patterns of barnacles from deep-sea hydrothermal vents. Mol Ecol; Feb;24(3), 673-89.
- Hou Y, Nowak MD, Mirré V, Bjorå CS, Brochmann C, Popp M. (2015) Thousands of RAD-seq Loci Fully Resolve the Phylogeny of the Highly Disjunct Arctic-Alpine Genus Diapensia (Diapensiaceae). PLoS One; Oct 8;10(10), e0140175.
- Gamble T, Coryell J, Ezaz T, Lynch J, Scantlebury DP, Zarkower D. (2015) Restriction Site-Associated DNA Sequencing (RAD-seq) Reveals an Extraordinary Number of Transitions among Gecko Sex-Determining Systems. Mol Biol Evol; May;32(5), 1296-309.
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Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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