The Monarch RNA Cleanup Kit (500 µg) enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).
Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
Specifications and Applications:
|RNA Sample Type||Cleanup of RNA from large-scale in vitro transcription reactions|
|Binding Capacity||500 µg|
|RNA Size Range||≥ 25 nt ( ≥ 15 nt with modified protocol)|
|Elution Volume||50–100 µl|
|Purity||A260/280 > 1.8 and A260/230 > 1.8|
|Protocol Time||10–15 minutes of spin and incubation time|
|Compatible Downstream Applications||RNA Labeling, RNAi, Microinjections, RT-PCR, RNA library prep for NGS, transfection|
|RNA Cleanup and Concentration (including from the TRIzol aqueous phase)||RNA purified by other methods can be further purified|
|Enzymatic Reaction Cleanup||Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting|
|In vitro Transcription Cleanup||Enzymes and excess NTPs are removed to yield highly pure synthesized RNA|
|RNA Gel Extraction||Purification of RNA from agarose gels|
|RNA Fractionation||Fractionation of RNA into small and large RNA pools|
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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