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The Monarch RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA cleanup and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after other purification methods such as phenol/chloroform extraction. The Monarch RNA Cleanup Kits are available in 3 different binding capacities: 10 μg (NEB #T2030), 50 μg (NEB #T2040) and 500 μg (NEB #T2050). Each kit contains unique columns, all designed to prevent buffer retention and ensure no carryover of contaminants, enabling low-volume elution of highly-pure RNA. Following the standard protocol, RNA ≥ 25 nt is purified with this kit; however, a modified protocol is available to enable the binding of RNA as small as 15 nt (including miRNAs).
Reasons to choose Monarch for RNA Cleanup
- Choose from 3 different binding capacities and elution volumes
- Quickly and easily purify large quantities of high-quality RNA from in vitro transcription (IVT) reactions
- Efficiently remove unincorporated nucleotides from your RNA sample
|RNA Cleanup and Concentration (including from the TRIzol aqueous phase)||RNA purified by other methods can be further purified|
|Enzymatic Reaction Cleanup||Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting|
|In vitroTranscription Cleanup||Enzymes and excess NTPs are removed to yield highly pure synthesized RNA|
|RNA Gel Extraction||Purification of RNA from agarose gels|
|RNA Fractionation||Fractionation of RNA into small and large RNA pools|
|Monarch RNA Cleanup Kit||NEB #T2030 (10 µg)||NEB #T2040 (50 µg)||NEB #T2050 (500 µg)|
|Binding Capacity:||10 μg||50 µg||500 µg|
|RNA Size Range:||≥ 25 nt ( ≥ 15 nt with modified protocol)|
|Elution Volume:||6–20 µl||20–50 µl||50–100 µl|
|Purity:||A260/280 > 1.8 and A260/230 > 1.8|
|Protocol Time:||5 minutes of spin and incubation time||10–15 minutes of spin and incubation time|
|Common Downstream Applications:||RT-PCR, RNA library prep for NGS, Small RNA library prep for NGS, RNA labeling||RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, transfection||RT-PCR, RNA library prep for NGS, RNA labeling, RNAi, microinjection, transfection|
Available kits include:
Learn how to use the Monarch RNA Cleanup Kits to quickly and easily purify RNA from enzymatic reactions including in vitro transcription reactions, labeling, and DNase I treatment.
Optimize the results of your RNA cleanups with these quick and simple tips.
Learn about the sources of RNase contamination and how you can prevent them from disrupting your experiments.