The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg, NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. They are supplied with Monarch Collection Tubes II (NEB #T2018). RNA Cleanup Binding Buffer (NEB #T2041) and Monarch RNA Wash Buffer (NEB #T2042) are also available separately.
Optimal for cleaning up RNA from a variety of enzymatic reactions
Ideal for the concentration of RNA into small volumes (elute in ≥ 6 µl)
70-100% RNA recovery, even with inputs <2 0 ng
Purified RNA is ready for use in a wide variety of downstream applications
Unique column design prevents buffer carryover and elution of silica particulates
Learn more about Monarch DNA and RNA purification products
The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg) (NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 6 μl and is ready for use in a variety of downstream applications including RT-PCR, RNA library prep for NGS, and labelling.
Monarch RNA Cleanup Columns are also available for 50 µg (NEB #T2047) and 500 µg (NEB #T2057) binding capacities.
Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl
rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.
Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl
A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.
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