Elution of large amounts of RNA (> 50 µg) can be maximized by incubating the elution buffer (nuclease-free water) with the column for 5 minutes at room temperature prior to spinning. Decreasing the incubation time will decrease RNA recovery.
Elution of large amounts of RNA (> 50 µg) can be maximized by incubating the elution buffer (nuclease-free water) with the column for 5 minutes at room temperature prior to spinning. Decreasing the incubation time will decrease RNA recovery.
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