PleI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10141295. Learn more.
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
Restriction Enzyme Cut Site: GAGTC(4/5)
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