BstEII

Companion Products

• BstEII-HF®
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• NEBuffer 3.1

1. Of the over 3,000 known restriction endonucleases, BstEII is one of the few that produces extensions of more than 4 bases.
2. May exhibit Star Activity in NEBuffer 2.1 and CutSmart Buffer.
3. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
4. Not sensitive to CpG, dcm, or dam methylation.
5. Star activity may result from a glycerol concentration of > 5%.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Dam and Dcm Methylases of E. coli
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Reduced Star Activities of HF® Enzymes
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Specificities
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Cross Index of Recognition Sequences
• Dam-Dcm and CpG Methylation
• Enzymes with Multiple Recognition Sequences
• Frequencies of Restriction Sites
• Interrupted Palindromes
• Isoschizomers
• Time-Saver™ Qualified Enzymes
• Why Choose Recombinant Enzymes?

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter V2.0
• NEBioCalculator
• REBASE

1. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
2. Why is my Restriction Enzyme not cutting DNA?
3. Why do I see a DNA smear on an agarose gel after a restriction digest?
4. Why do I see additional DNA bands on my gel after a restriction digest?
5. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?

• Restriction Enzyme Troubleshooting Guide

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0162S_L_v1
• R0162T_M_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0162S_L_v1_0851309
• R0162S_L_v1_0851405
• R0162S_L_v1_0841212
• R0162S_L_v1_0841304
• R0162T_M_v1_0841212
• R0162T_M_v1_0841304
• R0162T_M_v1_0851405
• R0162T_M_v1_0851410
• R0162S_L_v1_0851410
• R0162S_L_v1_0851504
• R0162T_M_v1_0851504
• R0162T_M_v1_0851509
• R0162S_L_v1_0851510
• R0162S_L_v1_0851604
• R0162T_M_v1_0851606
• R0162S_L_v1_0851610
• R0162T_M_v1_0851703
• R0162S_L_v1_0851709
• R0162T_M_v1_0851712
• R0162S_L_v1_0851804
• R0162L_v1_10008590
• R0162M_v1_10030264
• R0162L_v1_10028212
• R0162S_v1_10028214
• R0162S_v1_10031296
• R0162L_v1_10044563
• R0162M_v1_10043559
• R0162S_v1_10049593
• R0162L_v1_10052081
• R0162M_v1_10063036
• R0162L_v1_10065275
• R0162S_v1_10065273

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BstEII
• NEBuffer™ 3.1

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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