SacII

SacII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10180741. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence CC_GC^GG

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
Restriction Enzyme Cut Site: CCGC/GG

Catalog #	Concentration	Size	List Price	Your Price
R0157S	20,000 units/ml	2,000 units	$70.00	Sign In Sign In
R0157L	20,000 units/ml	10,000 units	$286.00	Sign In Sign In

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Product Information

Product Source

A Streptomyces lividans strain that carries the SacII gene from Streptomyces achromogenes (ATCC 12767).

This product is related to the following categories:: Restriction Endonucleases S Products,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0157S -20

SacII R0157SVIAL -20 1 x 0.1 ml 20,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

R0157L -20

SacII R0157LVIAL -20 1 x 0.5 ml 20,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. Certain SacII sites (e.g. one in the right arm of λ DNA) are resistant to cleavage. The reason particular SacII sites in λ DNA and ΦX174 DNA are cleaved at significantly lower rates than those found with other substrates is unclear at present.
2. SacII needs to interact with two copies of its recognition sequence to cleave. As a result, substrates with single SacII sites are cleaved at a reduced rate.
  
  For more information and recommendations on working with enzymes requiring multi-sites, please visit Restriction Enzyme Cleavage:‘single-site’ enzymes and ‘multi-site’ enzymes
3. Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
4. Blocked by CpG methylation.