TEV Protease

Features

• removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins
• engineered to improve thermal stability and decrease autolysis
• high substrate specificity with no non-specific proteolysis 

1. The optimum sequence for the TEV protease cleavage site is Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser). However, it has been reported that other amino acid residues can be used in the P1´ position instead of Ser or Gly. These alternative P1´ amino acids include Ala, Met, and Cys (1).
2. If the fusion protein sample contains > 2 M urea, > 0.5 M Guanidine hydrochloride, > 50 mM imidazole, pH values below 6 or above 9, or cysteine protease inhibitors then it will be necessary to dialyze the fusion protein before TEV Protease cleavage.
3. TEV protease can be used at high concentrations without the occurrence of non-specific proteolysis.
4. Reactions may be scaled-up linearly to accommodate larger reaction volumes.

1. Kapust, R.B. et al. (2002). Biochem. and Biophysical Research Comm.. 294, 949-955.

1. Typical Reaction Conditions for TEV Protease (NEB #P8112)
2. His-tag removal from protein using TEV Protease

• Protease Selection Chart

1. Can TEV Protease recognize and cleave a sequence other than Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)?
2. Is it necessary to dialyze the sample prior to cleavage with TEV Protease?
3. Can TEV Protease be used at lower temperatures?
4. Is TEV Protease compatible with protease inhibitors?
5. Is TEV Protease compatible with different reaction buffers?
6. Can TEV Protease be removed from the reaction after cleavage?

• Protein Expression & Purification Brochure
• Purification Beads, Columns and Resins Brochure

Product Citation Tool

• Over 40 years in protein expression and purification – a historical perspective

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P8112S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P8112S_v1_10017701
• P8112S_v1_10028767
• P8112S_v1_10031124
• P8112S_v1_10035078
• P8112S_v1_10037481
• P8112S_v1_10039748
• P8112S_v1_10045949
• P8112S_v1_10048134
• P8112S_v1_10049722
• P8112S_v1_10052586
• P8112S_v1_10055989
• P8112S_v1_10061036
• P8112S_v1_10064916
• P8112S_v1_10067995
• P8112S_v1_10073045
• P8112S_v1_10077415
• P8112S_v1_10081140
• P8112S_v1_10088321

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• TEV Protease
• TEV Protease Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.