Target Protein Insolubility
Choose Type:
- NEBExpress MBP Fusion and Purification System Quick Start Protocol (NEB #E8201)
- Cloning a PCR Fragment Into a pMAL Expression Vector (NEB #E8201)
- Transformation Protocol (NEB #E8201)
- Small Scale Affinity Chromatography (NEB #E8201)
- Large Scale Affinity Chromatography (NEB #E8201)
- Cleavage of the Fusion Protein
- Separating the Protein of Interest from MBP after TEV Protease Cleavage (NEB #E8201)
- NEBuilder Assembly of a PCR Fragment
- Transformation of SHuffle® Competent Cell Strains
- E coli Lemo21 DE3 A T7 RNA Polymerase-based protein overexpression platform for routine and difficult targets
- Protein Expression with T7 Express Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- DNA Sequences and Maps Tool
- Competent Cell Product Comparison
- Protein Expression and Purification Selection Chart
Feature Articles
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- Agrawal, A., Bisharyan, Y., Papoyan, A, Bednenko, J., Cardarelli, J., Yao, M., Clark, T., Berkmen, M., Ke, N., Colussi, P. (2019) Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli. Protein Expr Purif; 153, 7-17. PubMedID: 30081196, DOI: 10.1016/j.pep.2018.08.001.
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