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Protein Purification

New England Biolabs is committed to providing advanced tools for protein purification. Our NEBExpress^™ portfolio of products includes solutions for expression and purification and is supported by access to scientists with over 50 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.

Isolation of pure proteins for downstream experiments is a common, yet time-consuming, task. New England Biolabs offers a variety of resins and magnetic beads that are easy-to-use, highly specific, and available in several different formats for rapid isolation and purification of proteins. NEB’s magnetic beads are ideally suited for applications involving high-throughput proteomic screening and small-scale protein isolation. With magnetic beads, affinity purification of tagged proteins and antibodies can be done conveniently and quickly. NEB’s resins enable simple, one-step purification strategies for tagged proteins, and result in a high yield of highly pure protein. For the full list of products available for protein expression and purification, visit www.neb.com/ProteinExpression.

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Affinity Purification

Magnetic Beads and Racks

Affinity Purification

Amylose Purification (MBP-tag)

Nickel Purification (His-tag)

Chitin Purification (CBD-tag)

Magnetic Beads and Racks

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Protein Purification includes these subcategories:

Affinity Purification
Magnetic Beads and Racks

FAQs for Protein Purification

Protocols for Protein Purification

Application Notes for Protein Purification

Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress^® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads

Tools & Resources

Brochures

Competent Cell Brochure

Protein Expression & Purification Brochure

Purification Beads, Columns and Resins Brochure

Selection Tools

Affinity of Protein A/G for IgG Types from Different Species

Competent Cell Product Comparison

Protein Expression and Purification Selection Chart

Purification Beads, Columns and Resins

Posters

An E.coli lysate-based system for in vitro Protein Synthesis

Discovering the Maltose Binding Protein epitope tag for Escherichia coli expressed proteins (2018)

Selection for proteins that overcome heatinduced lethality of ΔdegP strain (2018)

Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)

NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)

Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Choose Product:

Affinity Purification

Magnetic Beads and Racks

Amylose Purification (MBP-tag)

Nickel Purification (His-tag)

Chitin Purification (CBD-tag)

Choose Type:

Brochures

Selection Tools

Posters

Featured Products

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