FAQ: Can TEV Protease recognize and cleave a sequence other than Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)?

The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQS. However, the amino acid in the P1’ position can also be G, A, M, C, or H (1)

  1. Kapust, R.B. et al. (2002) Biochem. and Biophysical Research Comm. 294, 949-955.