FAQ: Is it necessary to dialyze the sample prior to cleavage with TEV Protease?

If the fusion protein sample contains >2 M urea, >0.5 M guanidine hydrochloride, >50 mM imidazole, pH values below 6 or above 9, or cysteine protease inhibitors, then it will be necessary to dialyze the fusion protein before TEV Protease cleavage.