RNase Inhibitor, Murine
RNase Inhibitor, Murine, specifically inhibits RNases A, B and C.
- Improved resistance to oxidation, compared to human/porcine RNase inhibitor
- Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
- Isolated from a recombinant source
- Tested for the absence of DNases and RNases
Avoiding RNase Contamination
RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
Recombinant murine RNase inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Product SourceAn E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.
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