T4 DNA Ligase
- The industry standard for performance and quality
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
What are the best conditions for DNA ligation?
What molar ratios should I use for DNA Ligation?
How do I choose the best DNA Ligase?
Why do I need to add PEG to my DNA ligation?
Traditional Cloning Workflow
Overview of PCR Cloning
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1). T4 DNA ligase will seal nicks for these DNA substrates.
For details on NEB's quality controls for DNA ligases, visit our Ligase Quality page.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Product SourcePurified from E. coli C600 pcl857 pPLc28 lig8 (2).
The following reagents are supplied with this product:
Store at (°C) Concentration T4 DNA Ligase Reaction Buffer -20 10 X
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