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Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Enzyme Activity in rCutSmart Buffer Required Supplements*
Antarctic Phosphatase +++  Requires Zn2+
Authenticase ++  
Bst DNA Polymerase +++  
CpG Methyltransferase (M. SssI) +++  
DNA Polymerase I +++  
DNA Polymerase I, Large (Klenow) Fragment
 +++  
DNA Polymerase Klenow Exo- +++  
DNase I (RNase-free) +++ Requires Ca2+
DNase I-XT
  +++ Requires Ca2+
Duplex DNase +  
E. coli DNA Ligase +++ Requires NAD
Exonuclease I
  +++  
Thermolabile Exonuclease I
  +++  
Exonuclease III +++  
Endonuclease III (Nth), recombinant +++  
Exonuclease V (Rec BCD)  +++ Requires ATP
Exonuclease VII +++
 
Endonuclease VIII  +++  
Fpg  +++   
GpC Methyltransferase (M. CviPI) + Requires DTT
Lambda Exonuclease  ++  
Micrococcal Nuclease +++ Requires Ca2+
Mismatch EndoI ++  
Thermostable EndoQ*
 ++   
Thermostable OGG*
 +++   
phi29 DNA Polymerase +++ Requires DTT
RecJf +++  
rSAP +++  
Quick CIP
 +++   
T3 DNA Ligase +++ Requires ATP + PEG
T4 DNA Ligase +++ Requires ATP
Hi-T4™ DNA Ligase +++ Requires ATP
Salt-T4® DNA Ligase + Requires ATP
T4 DNA Polymerase +++  
T4 Phage β-glucosyltransferase (T4-BGT) +++  
T4 Polynucleotide Kinase +++ Requires ATP + DTT
T4 PNK (3 ' phosphatase minus) +++ Requires ATP + DTT
T5 Exonuclease  +++  
T7 DNA Ligase +++ Requires ATP + PEG
T7 DNA Polymerase (unmodified) +++
T7 Exonuclease +++  
USER® Enzyme, recombinant
 +++  
Thermolabile USER II
 +++   
Thermostable USER III
  ++  
Warmstart Afu UDG +  

*Refer to “Reaction Conditions” on product webpage for recommended supplement concentration.

+++ full functional activity
++ 50-100% functional activity
+ 0-50% functional activity