Epigenetics
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  • Epigenetics

    New England Biolabs has called upon its 35 years of expertise in enzymology to develop a suite of validated reagents for epigenetics research. This line of easy-to-use EpiMark® kits simplifies DNA methylation and hydroxymethylation detection and analysis, as well as ChIP, histone and nucleosome analysis. Independently applicable, individual epigenetics reagents also complement the EpiMark® kits. NEB's methylation- and hydroxymethylation- sensitive or dependent enzymes, DNA methyltransferases and DNA controls are all useful for mapping DNA modifications and methylating DNA at specific sites for gene expression studies. Our protein methyltransferases and recombinant histones perform efficiently in protein modification and characterization studies. Our range of modified and unmodified genomic DNAs can be used as controls for detection of DNA methylation. Our series of human DNA (cytosine-5) methyltransferase (DNMT) antibodies are ideally suited for Western blots and immunoprecipitation.

    Our complete Epigenetics suite is expertly designed for optimized research and discovery.

    EpiMark® is a registered trademark of New England Biolabs, Inc.
    1. What Is Epigenetics?

      If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

    Learn More

    Epigenetics includes these subcategories:

    Antibodies Information

    Control DNA Information
    Epigenetic Analysis (Epimark® Validated) Information
    Histones Information
    Methylation Dependent Restriction Enzymes for Epigenetics
    Methylation Sensitive Restriction Enzymes for Epigenetics
    Methyltransferases for Epigenetics

      Publications related to Epigenetics:

    1. Marx V. (2016). Genetics: profiling DNA methylation and beyond Nature Methods. 13, 119-122. DOI: 10.1038/nmeth.3736
    2. Chernov AV., Reyes L., Peterson S., Strongin AY. (2015). Depletion of CG-Specific Methylation in Mycoplasma hyorhinis Genomic DNA after Host Cell Invasion PLoS One. 10, e0142529. PubMedID: 26544880, DOI: 10.1371/journal.pone.0142529
    3. Wee E., Ngo T., Trau M. (2015). A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation Sci Rep. 5, 15028. PubMedID: 26458746, DOI: 10.1038/srep15028
    4. Kienhöfer S., Musheev M., Stapf U., Helm M., Schomacher L., Niehrs C., Schäfer A. (2015). GADD45a physically and functionally interacts with TET1Publication Differentiation. PubMedID: 26546041, DOI: 10.1016/j.diff.2015.10.003
    5. Page A., Paoli P., Salvador E., White S., French J., Mann J. (2015). Hepatic Stellate Cell Transdifferentiation Involves Genome-Wide Remodeling of the DNA Methylation Landscape J Hepatol. PubMedID: 26632634, DOI: 10.1016/j.jhep.2015.11.024
    6. Grant, T.J., et al. (2012). Antiproliferative small-molecule inhibitors of transcription factor LSF reveal oncogene addiction to LSF in hepatocellular carcinoma Proc. Natl. Acad. Sci. USA. 109, 4503-4508. PubMedID: 22396589
    7. Gu, L.Q., et al. (2012). Detection of miRNAs with a nanopore single-molecule counter Expert Rev. Mol. Diagn.. 12, 573-584. PubMedID: 22845478
    8. Ho, J.J., et al. (2012). Functional importance of Dicer protein in the adaptive cellular response to hypoxia J. Biol. Chem.. 17, 29003-20. PubMedID: 22745131, DOI: 10.174/jbcM112.373365
    9. Gong, H. wt al. (2012). Near-infrared fluorescence imaging of mammalian cells and xenograft tumors with SNAP-tag PLoS ONE. 7, PubMedID: 22479502
    10. Sexton T, Kurukuti S, Mitchell JA, Umlauf D, Nagano T, Fraser P (2012). Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip Nat Protoc. 7(7), 1335-50. PubMedID: 22722369, DOI: 10.1038/nprot.2012.071
    11. Stroud, H., et al. (2011). 5-Hydroxymethylcytosine is associated with enhancers and gene bodies in human embryonic stem cells Gen. Biol.. PubMedID: 21689397
    12. Canc. Res. (2011). 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. 71, 1904-1911. PubMedID: 21239472
    13. (2011). Comparitive characterization of the PvuRts11 family and application in mapping genomic 5-hydroxymethylcytosine Nucl. Acids Res.. 39, 9294-9305. PubMedID: 21813453, DOI: 10.1093/nar/gkr607
    14. Zhang, J. et al. (2011). Cyclophosphamide perturbs cytosine methylation in jurkat-T Cells through LSD1-mediated stabilization of DNMT1 Protein Chem. Res. Toxicol.. 24(11), 2040-2043. PubMedID: 22007908
    15. Ficz, G., et al. (2011). Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation Nature . PubMedID: 21460836, DOI: 10.1038/nature10008
    16. Diep, D. and Zhang, K. (2011). Genome-wide mapping of the sixth base Genome Biol. . 12, 116. PubMedID: 21682934, DOI: 10.1186/gb-2010-12-6-116
    17. Wolff, E.M. et al. (2011). Hypomethylation of a LINE-1 Promoter Activates an Alternate Transcript of the MET Oncogene in Bladders with Cancer PLoS Genet. . 6, 4:e1000917. PubMedID: 20421991, DOI: 10.1371/journal.pgen.1000917
    18. Nelson, F.K, et. al. (2011). Introduction and historical overview of DNA sequencing Curr. Prot. Mol. Biol.. Unit 7.0.1-7.0.18,
    19. Cohen-Karni, D, et al. (2011). The MspJI family of modification-dependent restriction endonucleases for epigenetic studies Proc. Natl. Acad. Sci. . PubMedID: 21690366, DOI: 10.1073/pnas.1018448108
    20. Kinney, S.M. et al. (2011). Tissue specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genome J. Biol. Chem. . PubMedID: 21610077, DOI: 10.1074/jbc.m110.217083
    21. Zheng, Y. et al. (2010). A unique family of Mrr-like modification-dependent restriction endonucleases Nucl. Acids Res.. 38(16), 5527-5534. PubMedID: 20444879
    22. Wanunu, M. et al. (2010). Discrimination of methylcytosine from hydroxymethylcytosine in DNA molecular J. Am. Chem. Soc.. 133(3), 486-492. PubMedID: 21155562
    23. Jensen, H.M., et al. (2010). Engineering of a synthetic electron conduit in living cells Proc. Natl. Acad. Sci. USA. 107, 19213-19218. PubMedID: 20956333
    24. Wanunu, M., et al. (2010). Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors Nature Nanotech. 5, 807-814. PubMedID: 20972437
    25. Laget, S., et al. (2010). The human proteins MBD5 and MBD6 associate with heterochromatin but they do not bind methylated DNA PLoS One. 5, PubMedID: 20700456

    Types of Histone Modifications

    Amino Acid Modification
    Lysine Methylation, Acetylation,
    Ubiquitination, Sumoylation,
    ADP-Ribosylation
    Arginine Methylation
    Serine Phosphorylation
    Threonine Phosphorylation

    Types of DNA Modifications

    References

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    2. Vanyushin, B.F. (2006) Curr. Top. Microbiol. Immunol. 301, 67-122. PMID: 16570846
    3. Mosher, R.A., Melnyk, C.W. (2010) Trends Plant Sci. 15, 204-210. PMID: 20129810
    4. Lyko, F., Beisel, C., Marhold, J., Paro, R. (2006) Curr. Top. Microbiol. Immunol. 310, 23-44. PMID: 16909905
    5. Selker, E.U., Freitag, M., Kothe, G.O., et al. (2002) Proc. Natl. Acad. Sci. U S A. 99, Suppl 4, 16485-16490. PMID: 12189210
    6. Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-930. PMID: 19372393
    7. Tahiliani, M., Koh, K. P., Shen, Y., et al. (2009) Science 324, 930-935. PMID: 19372391
    8. Ehrlich, M., Wilson, G.G., Kuo, K.C., And Gehrke, C.W. (1987) J. Bacteriol. 169, 939-943. PMID: 3029036
    9. Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A. and Matvienko, N.I. (2004) Biochem. (Moscow) 69, 299-305. PMID: 15061697
    10. Ratel, D., Ravanat, J-L., Berger, F. and Wion D. (2006) Bioessays 28, 309-315. PMID: 16479578
    11. Marinus, M.G. and Casadesus, J. (2009) FEMS Microbiol. Rev. 33, 488-503. PMID: 19175412

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