Do you prefer to use non-strand-specific library prep methods, but need increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA? To address these challenges, our next generation of non-directional RNA library prep kit has been reformulated at each step, resulting in several fold higher yields of high quality libraries and enabling use of lower input amounts and fewer PCR cycles.
10 ng – 1 µg Total RNA (polyA mRNA workflow)
5 ng – 1 µg Total RNA (rRNA depletion workflow)
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
For directional (strand-specific) RNA library preparation, see the NEBNext Ultra II Directional RNA Library Prep kit for Illumina.
Also available without optional SPRIselect® beads.
For extensive NEBNext Ultra II performance data, click the links in the Features above and download our technical note for poly(A) mRNA isolation or our technical note for rRNA depletion.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Ligation Enhancer||-20|
|NEBNext First Strand Synthesis Reaction Buffer||-20|
|NEBNext Second Strand Synthesis Enzyme Mix||-20|
|NEBNext Second Strand Synthesis Reaction Buffer||-20||10 X|
|NEBNext Ultra II End Prep Enzyme Mix||-20|
|NEBNext Ultra II End Prep Reaction Buffer||-20|
|NEBNext® Ultra™ II Ligation Master Mix||-20|
|NEBNext® Ultra II Q5® Master Mix||-20||2 X|
|NEBNext First Strand Synthesis Enzyme Mix||-20|
|NEBNext Adaptor Dilution Buffer||-20||1 X|
|(0.1X) TE Buffer||-20||0.1 X|
|NEBNext® Sample Purification Beads||25|
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