The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
For directional (strand-specific) RNA library preparation, see the NEBNext Ultra II Directional RNA Library Prep kit for Illumina.
Also available with optional SPRIselect® beads.
For extensive NEBNext Ultra II performance data, click the links in the Features above and download our technical note for poly(A) mRNA isolation or our technical note for rRNA depletion.
View additional data on library yields
View additional data on library quality
MAXIMIZING TRANSCRIPT COVERAGE
View additional data on transcript coverage
EXCELLENT LIBRARY COMPLEXITY AT LOW INPUT AMOUNTS
View additional data on library complexity
The following reagents are supplied with this product:
|Store at (°C)||Concentration
|NEBNext Ligation Enhancer||-20||
|NEBNext First Strand Synthesis Reaction Buffer||-20||
|NEBNext Second Strand Synthesis Enzyme Mix||-20||
|NEBNext Second Strand Synthesis Reaction Buffer||-20||10X
|NEBNext Ultra II End Prep Enzyme Mix||-20||
|NEBNext Ultra II End Prep Reaction Buffer||-20||
|NEBNext Ultra II Ligation Master Mix||-20||
|NEBNext® Ultra II Q5® Master Mix||-20||2X
|NEBNext First Strand Synthesis Enzyme Mix||-20||
|NEBNext Adaptor Dilution Buffer||-20||
|(0.1X) TE Buffer||-20||0.1X