NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes a methylated version of the NEBNext adaptor, which is compatible with library preparation for bisulfite sequencing protocols.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Compatible with bisulfite sequencing protocols

Ordering Information

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  • 24 reactions
    $115.00
  • 96 reactions
    $420.00
  • Product Information

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    Incorporating a novel hairpin loop structure, the NEBNext adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

    NEBNext oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

    For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact custom@neb.com for further information.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext Index 12 Primer for Illumina -20
    NEBNext Universal PCR Primer for Illumina
    USER® Enzyme -20
    NEBNext Index 1 Primer for Illumina -20
    NEBNext Index 2 Primer for Illumina -20
    NEBNext Index 3 Primer for Illumina -20
    NEBNext Index 4 Primer for Illumina -20
    NEBNext Index 5 Primer for Illumina -20
    NEBNext Index 6 Primer for Illumina -20
    NEBNext Index 7 Primer for Illumina -20
    NEBNext Index 8 Primer for Illumina -20
    NEBNext Index 9 Primer for Illumina -20
    NEBNext Index 10 Primer for Illumina -20
    NEBNext Index 11 Primer for Illumina -20
    NEBNext Methylated Adaptor for Illumina -20
    Product Categories:
    Adaptors and Primers for Library Prep
    Applications:
    RNA-seq,
    rna-for-illumina,
    illumina-library-preparation
    ,
    Applications of USER® and Thermolabile USER II Enzymes
    • Properties and Usage
  • FAQs & Tech Tips
  • Protocols & Manuals
  • Other Tools & Resources
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    • Quality Control Assays
    • Certificate of Analysis
    • Safety Data Sheets
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