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  • NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

    Description

    The NEBNext Multiplex Oligos for Illumina (Methylated Adaptor, Index Primers Set 1) contains adaptors and primers that are ideally suited for multiplex sample preparation for whole genome bisulfite sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

    Functionally Validated
    Each set of reagents is functionally validated together through construction and sequencing of bisulfite converted genomic DNA libraries on the Illumina platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    USER Enzyme
    NEBNext Universal PCR Primer for Illumina-20
    NEBNext Index 1 Primer for Illumina
    NEBNext Index 2 Primer for Illumina
    NEBNext Index 3 Primer for Illumina
    NEBNext Index 4 Primer for Illumina
    NEBNext Index 5 Primer for Illumina
    NEBNext Index 6 Primer for Illumina
    NEBNext Index 7 Primer for Illumina
    NEBNext Index 8 Primer for Illumina
    NEBNext Index 9 Primer for Illumina
    NEBNext Index 10 Primer for Illumina
    NEBNext Index 11 Primer for Illumina
    NEBNext Index 12 Primer for Illumina
    NEBNext Methylated Adaptor for Illumina

    Properties and Usage

    Materials Required but not Supplied

    Compatible with the following library preparation kits:
    NEBNext Ultra™ DNA Library Prep Kit for Illumina (#E7370),
    NEBNext DNA Library Prep Master Mix Set for Illumina (#E6040), or
    NEBNext DNA Library Prep Reagent Set for Illumina (#E6000)

    NEB EpiMark® Bisulfite Conversion Kit (NEB # E3318S)
    NEB EpiMark Hot Start Taq DNA Polymerase (NEB # M0490S)

    10 mM dNTPs
    Freshly prepared 80% Ethanol
    Magnetic Stand
    DNA LoBind® Tubes (Eppendorf®)/PCR Plates
    Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
    Bioanalyzer® (Agilent® Technologies, Inc.)
    Nuclease Free Water
    0.1X TE (or 10 mM Tris-HCl, pH 8.0)

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
    1. What are the major applications of NEBNext Methylated Adaptor for Illumina?
    2. Is library prep workflow different when NEBNext Methylated Adaptor is used?
    3. How is NEBNext Methylated Adaptor (NEB #E7535) different from NEBNext adaptor for Illumina (NEB #E7335 & #E7350)?
    1. Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (#E7370)
    2. Protocol for use with NEBNext DNA Library Prep Master Mix Set for Illumina (#E6040)
    3. Protocol for use with NEBNext DNA Library Prep Reagent Set for Illumina (#E6000)