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NEBNext® DNA Library Prep Master Mix Set for Illumina®

In place of E6040 (discontinued on January 02 2020), the following products can be used together, in the same protocol:
NEBNext® End Repair Module (NEB #E6050)
NEBNext® dA-Tailing Module (NEB # E6053)
NEBNext® Quick Ligation Module (NEB #E6056)
NEBNext® Q5® HotStart HiFi PCR Master Mix (NEB #M0543)
Please note that for DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645,#E7103) or the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805, #E6177)
  • Catalog # E6040 was discontinued on January 02, 2020 ;
This kit utilizes the original standard library prep workflow, which is longer than the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645) workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow.
Learn more about how this product is being used in the Product Citation Tool
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