NEBNext® DNA Library Prep Master Mix Set for Illumina®

Companion Products

• NEBNext® End Repair Module
• NEBNext® dA-Tailing Module
• NEBNext® Quick Ligation Module
• NEBNext® dsDNA Fragmentase®

1. For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

1. What are the differences between expression library preparation vs. sample preparation suitable for the Illumina platform?
2. Can NEB provide any of the reagents for the cluster generation step or the actual sequencing run?
3. Are these reagents available in a customized format or bulk format?
4. What type of sample prep/library construction can I use NEBNext DNA Library Prep Master Mix Set for Illumina for?
5. What type and how much starting material do I need to use when preparing libraries using the NEBNext DNA Library Prep Reagent Set for Illumina?
6. Can I use the NEBNext DNA Library Prep Master Mix Set for Illumina reagents for preparing RNA? For miRNA work?
7. Can I use the NEBNext DNA Library Prep Master Mix Set for Illumina reagents for preparing libraries for sequencing on the Roche 454 GS FLX ?
8. Can I use the NEBNext DNA Library Prep Master Mix Set for Illumina reagents for preparing libraries for sequencing on the ABI SOLiD instrument?
9. How do I know the concentration of my index primers? 

1. Protocol for use with NEBNext DNA Library Prep Master Mix Set for Illumina (E6040)

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E6040S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E6040S_v1_10013246
• E6040L_v1_10018248
• E6040L_v1_10028724
• E6040S_v1_10026801
• E6040S_v1_10031619
• E6040L_v1_10022114

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext End Repair Enzyme Mix
• NEBNext End Repair Reaction Buffer
• Klenow Fragment (3´→ 5´ exo^–)
• NEBNext dA- Tailing Reaction Buffer
• Quick T4 DNA Ligase
• NEBNext Quick Ligation Reaction Buffer
• NEBNext® Q5® Hot Start HiFi PCR Master Mix

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

This product is covered by one or more patents.

This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.

• This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.