EpiMark® Bisulfite Conversion Kit

Bisulfite conversion is the most commonly used technique to date and is the “gold standard” for methylation analysis.

  • Incubation of the target DNA with sodium bisulfite results in conversion of all unmodified cytosines to uracils leaving the modified bases (5-mC or 5-hmC) intact
  • Complete conversion of Cytosine is achieved by alternating cycles of thermal denaturation with incubation reactions
  • This kit provides materials for 48 reactions
Catalog # Concentration Size List Price Your Price Quantity
E3318S Not Applicable 48 reactions $175.00
Please enter a quantity for at least one size
  • Product Information
    Genomic DNA from many organisms has modified nucleotides. In the mammalian genome, the modified base is predominately 5-methylcytosine (5-mC), which is involved in gene expression regulation, including selective inactivation of one X chromosome in females of mammalian species (1,2). Symmetrical CpG DNA methylation is heritable, but also reversible. This change of methylation status results in an enormous number of combinations of epigenetic states that can regulate gene expression. In mammalian cells, DNA methylation mainly occurs in CpG dinucleotides and is carried out by two methyltransferase enzymatic activities, namely, maintenance methylation and de novo methylation. The maintenance methyltransferase, DNMT1, is involved in the DNA methylation after every cellular DNA replication cycle. DNMT3a and DNMT3b are the de novo methyltransferases that are active in early development.

    For optimal results, 50 ng–2 μg DNA is recommended.

    Kit Components: 
    Each EpiMark Bisulfite Conversion Kit contains sufficient reagents to perform the bisulfite conversion reaction of 48 samples. Store at room temperature (15–20°C). However, for long-term storage (longer than 4 weeks) Desulphonation Reaction Buffer concentrate should be stored at 2–8°C. Prepared Bisulfite Mix can be stored at -20°C for up to six months. 

    Kit Components

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    • E3318S     25    
    Product Categories:
    Epigenetic Analysis
    Bisulfite Conversion
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