The DNA Library Prep Reagent Set for Illumina contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Phosphorylation Reaction Buffer -20 10 X Deoxynucleotide Solution Mix -20 10 mM T4 DNA Polymerase -20 DNA Polymerase I, Large (Klenow) Fragment -20 T4 Polynucleotide Kinase -20 Deoxyadenosine 5´- Triphosphate (dATP) -20 1 mM Klenow Fragment (3´→ 5´ exo–) -20 NEBuffer 2 for Klenow Fragment (3´→ 5´ exo–) -20 10 X Quick T4 DNA Ligase -20 NEBNext® Quick Ligation Reaction Buffer -20 2 X
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