EpiMark® Hot Start Taq DNA Polymerase
Taq DNA Polymerase optimized for epigenetics analysis
- Ideal for use on bisulfite-converted DNA and AT-rich templates
- Specially formulated reaction buffer system
- Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA.
Taq DNA polymerase possesses a 5´- 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
The following reagents are supplied with this product:
Store at (°C) Concentration EpiMark® Hot Start Taq Reaction Buffer Pack -20 5X
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.