NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®
Biomek Automated NEBNext Ultra Directional RNA Library Prep Kit for Illumina
The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® contains reagents for preparation of strand-specific RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion reagents and poly(A) mRNA isolation reagents are not included in the kit and are available separately.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext End Prep Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X Blunt/TA Ligase Master Mix -20 NEBNext First Strand Synthesis Reaction Buffer -20 Random Primers -20 ProtoScript® II Reverse Transcriptase -20 200,000 units/ml Murine RNase Inhibitor -20 NEBNext Second Strand Synthesis Enzyme Mix -20 NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix -20 10 X NEBNext USER® Enzyme -20 Nuclease-free Water -20 NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
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