NEBNext® Singleplex Oligos for Illumina®

Description






The NEBNext Singleplex Oligos for Illumina contains adaptors and primers that are ideally suited for sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

Figure 1: Workflow
Figure 1: Workflow

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
USER® Enzyme-20
NEBNext Universal PCR Primer for Illumina
NEBNext Index 1 Primer for Illumina
NEBNext Adaptor for Illumina

Properties and Usage

Materials Required but not Supplied

  • Enzymes and buffers appropriate for DNA, CHiP or mRNA Library Preparation (NEB #E6000, E6040, E6056, E6100, E6110, E6200, E6240, E7420, E7445, E7530, E7370)
  • AMPure® XP Beads (Beckman Coulter, Inc.) or PCR Column Purification Kit (Qiagen or other)
  • Size Selection Materials [E-Gel® (Life Technologies, Inc.), Agarose Gel or AMPure XP Beads]
  • DNA LoBind® Tubes (Eppendorf®)/PCR Plates
  • Magnetic Stand
  • Bioanalyzer® (Agilent Technologies, Inc.)
  • Freshly Prepared 80% Ethanol
Please Refer to the Kit Specific Protocol for using the NEBNext Singleplex Oligos for Illumina:

The following kits are designed for use with the NEBNext Singleplex Oligos for Illumina:
  • #E7420, NEBNext Ultra Directional RNA Library Prep Kit for Illumina
  • #E7530, NEBNext Ultra RNA Library Prep Kit for Illumina
  • #E7370, NEBNext Ultra DNA Library Prep Kit for Illumina
  • #E7445, NEBNext Ultra Ligation Module
  • #E6040, NEBNext DNA Library Prep Master Mix Set for Illumina
  • #E6110, NEBNext mRNA Library Prep Master Mix Set for Illumina
  • #E6240, NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
  • #E6056, NEBNext Quick Ligation Module
  • #E6000, NEBNext DNA Library Prep Reagent Set for Illumina
  • #E6200, NEBNext ChIP-Seq Library Prep Reagent Set for Illumina
  • #E6100, NEBNext mRNA Library Prep Reagent Set for Illumina

Storage Temperature

-20°C

FAQs

  1. Are NEBNext adaptor and primers for Illumina validated in Next Generation Sequencing Workflows?
  2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
  3. Is USER® enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
  4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
  5. Are these reagents available in a customized format or bulk format?
  6. Can USER® Enzyme be purchased separately?
  7. Are the NEBNext Oligos for Illumina compatible with single end and paired end sequencing?
  8. Is the USER® enzyme in our NEBNext kits identical in concentration to the standalone product?

Protocols

  1. Please see manual (NEB #E7350) for protocols

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.