NEBNext® Singleplex Oligos for Illumina®
NEBNext® Singleplex Oligos provide adaptors and primers to enable high yield singleplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation. The primers provided enable construction of singleplex libraries, or the universal PCR primer provided can be used in conjunction with user-supplied index primers to prepare multiplex libraries.
Note that NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
- High efficiency adaptor ligation
- Minimized adaptor-dimer formation
- Suitable for singleplex library construction, or for use with user-supplied index primers
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, user-supplied barcodes can be incorporated, thereby enabling multiplexing. Note that the PCR primers supplied in the kit enable production of singleplex libraries only.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Multiplex single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Universal PCR Primer for Illumina NEBNext Adaptor for Illumina -20 USER® Enzyme -20 NEBNext Index 1 Primer for Illumina
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