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All of NEB's enzymes undergo extensive quality controls to ensure purity and lot-to-lot consistency. NEB glycosidases, including PNGase F products, are no different, and are tested for contaminating exoglycosidase, endoglycosidase and proteolytic activities. Unlike other suppliers, NEB uses fluorescently-labeled oligosaccharides to screen for contaminating activities, which increases sensitivity. Consistency and reproducibility are tested across three lots, ensuring optimized activity for your application.
 
 

Highlights

NEB is certified to both ISO 9001:2008 and ISO 13485:2003 standards for quality management systems.

ISO_9001_PNGaseF  ISO_13485_PNGaseF
  • Recombinant enzymes; manufactured in-house
  • All glycosidase products from NEB exhibit ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Stringent quality control testing includes assays for detecting contaminating exoglycosidase, endoglycosidase and proteolytic activity

Quality data

Every NEB enzyme is subjected to a quality control assay to determine protein purity. The physical purity is assessed by both SDS-PAGE and intact ESI-MS analysis. An example of this is shown below, using PNGase F. It is imperative to hold these enzymes to a high quality standard, as they must be compatible with the needs of regulated applications and standardized procedures, as well being free of contaminants and side activities. High-quality products are also necessary to meet challenges associated with glycoproteomic analyses.


Purity determination by SDS-PAGE and ESI-MS analysis
PNGaseF_LP_quality_data
(A) Protein Purity of PNGase F is compared across product types and production lots.
Lane 1: 5 μl Color Prestained Protein Standard (11-245 kDa) (NEB #P7712)
Lane 2: 5 μl PNGase F (Glycerol Free), Lot 41 (NEB #P0705)
Lane 3: 5 μl PNGase F (Glycerol Free), Recombinant, Lot 2 (NEB #P0709)
Lane 4: 5 μl PNGase F (Glycerol Free), Recombinant, Lot 3 Lane 5: 5 μl PNGase F (Glycerol Free), Recombinant, Lot 4

(B) PNGase F (Glycerol Free), Recombinant (NEB# P0709), Lot 2. MW: 34,906.53 Daltons. Mass determination by an Agilent 6210 TOF LC/MS.
 
 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain application.

Available PNGase F products:

NEBNext_portal_product_icon  Rapid PNGase F 
  • Rapid PNGase F (NEB #P0710) completely deglycosylates antibodies and immunoglobulin fusion proteins in 10 minutes
  • Rapid PNGase F (non-reducing format) (NEB #P0711) completely deglycosylates in 10 minutes, while preserving disulfide bonds and quaternary structure for intact antibody analysis
  • Compatible with downstream HPLC and mass spectrometry analysis
  • Recombinant enzyme, releases N- glycans rapidly and without bias

 

NEBNext_portal_product_icon  PNGase F, Recombinant 

 

NEBNext_portal_product_icon  Remove-iT™ PNGase F 
  • Remove-iT PNGase F (NEB #P0706) is CBD-tagged for easy removal of the enzyme by incubation with chitin magnetic beads
  • Identical specificity and activity as non-tagged PNGase F

 

NEBNext_portal_product_icon  PNGase F