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Harvard Medical School BPF NEBnow

My NEB Freezer Location and Hours


Location:
Biopolymers Facilties Self Service Supply Center
142 Goldenson Building, 200 Longwood Avenue
Boston, MA 02115

Hours:
NEBnow® Kiosk, 24 x 7 access

Contact:
James Caffery
Phone: (617) 432-7480
Fax:(617) 432-7440
freezer@genome.med.harvard.edu

NEBNow Purchasing From Freezer
NEBnow® Purchasing from Your NEB Product Freezer

Learn how to purchase products from an NEBnow freezer. It’s as quick and easy as 1, 2, 3…

joanna_hamilton
My NEB Academic Accounts

Joanna Hamilton, Ph.D.
Phone: 978-471-2382
jhamilton@neb.com


Learn more about your NEBnow freezer


NEBnow_FreezerPage_Products_Icon  

Products Available in this Freezer


Inventory is replenished every Wednesday. See below for the regularly stocked product list and their estimated availability as of 12/6/2021 1:30 AM.

Are you interested having in an NEB product added to the regularly stocked inventory? Please tell us which product(s) and we'll contact your NEB Account Manager about your request.

NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 2
B7204S CutSmart® Buffer 5 ml 2
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
B7021S Gel Loading Dye, Blue (6X) 4 ml 2
B9004S ThermoPol® Reaction Buffer Pack 6 ml 2
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Quantity Available
E2611L Gibson Assembly® Master Mix 50 reactions 3
E2611S Gibson Assembly® Master Mix 10 reactions 3
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 3
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 2
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions 4
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
M0289S Antarctic Phosphatase 1,000 units 2
M0293L Exonuclease I (E. coli) 15,000 units 2
M0293S Exonuclease I (E. coli) 3,000 units 2
M0370S Instant Sticky-end Ligase Master Mix 50 reactions 2
M0212S Klenow Fragment (3'→5' exo-) 200 units 1
M0247S Micrococcal Nuclease 320,000 gel units 2
M2200L Quick Ligation™ Kit 150 reactions 3
M2200S Quick Ligation™ Kit 30 reactions 3
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 2
M0202L T4 DNA Ligase 100,000 units 3
M0202T T4 DNA Ligase 20,000 units 2
M0202M T4 DNA Ligase 100,000 units 2
M0202S T4 DNA Ligase 20,000 units 4
B0202S T4 DNA Ligase Reaction Buffer 6 ml 2
M0201S T4 Polynucleotide Kinase 500 units 3
M0201L T4 Polynucleotide Kinase 2,500 units 1
M0208S Taq DNA Ligase 2,000 units 2
M0208L Taq DNA Ligase 10,000 units out of stock
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 3
R0176L DpnI 5,000 units 2
R3138S SalI-HF® 2,000 units 2
NEB # Markers & Ladders Size Quantity Available
N3232L 1 kb DNA Ladder 1,000 gel lanes (1 ml) 2
N3232S 1 kb DNA Ladder 200 gel lanes (0.2 ml) 2
N3200L 1 kb Plus DNA Ladder 1,000 gel lanes (0.5 ml) 3
N3200S 1 kb Plus DNA Ladder 200 gel lanes (0.1 ml) 3
N3231S 100 bp DNA Ladder 100 gel lanes (0.1 ml) 3
N3231L 100 bp DNA Ladder 500 gel lanes (0.5 ml) 3
N0468S Quick-Load® 1 kb DNA Ladder 125 gel lanes (1.25 ml) 2
N0467S Quick-Load® 100 bp DNA Ladder 125 gel lanes (1.25 ml) 2
N0552S Quick-Load® Purple 1 kb DNA Ladder 125 gel lanes (1.25 ml) 3
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 250 gel lanes (1.25 ml) 1
N0551S Quick-Load® Purple 100 bp DNA Ladder 125 gel lanes (1.25 ml) 1
NEB # Nucleic Acid Purification Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 3
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps 2
T1034L Monarch® DNA Cleanup Columns (5 μg) 100 columns 1
T1020L Monarch® DNA Gel Extraction Kit 250 preps 2
T1020S Monarch® DNA Gel Extraction Kit 50 preps 3
T3010L Monarch® Genomic DNA Purification Kit 150 preps 2
T3010S Monarch® Genomic DNA Purification Kit 50 preps 2
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps 2
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 3
T1010S Monarch® Plasmid Miniprep Kit  50 preps 2
T1010L Monarch® Plasmid Miniprep Kit  250 preps 2
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
P0756S Adenosine 5'-Triphosphate (ATP) 1 ml 1
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol 3
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol 3
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions 2
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions 2
E3006L Luna® Universal Probe One-Step RT-qPCR Kit 500 reactions 2
M3004S Luna® Universal Probe qPCR Master Mix 200 reactions 2
M3004L Luna® Universal Probe qPCR Master Mix 500 reactions 2
M3003S Luna® Universal qPCR Master Mix 200 reactions 2
M3003L Luna® Universal qPCR Master Mix 500 reactions 2
E3010S LunaScript® RT SuperMix Kit 25 reactions 1
M0543S NEBNext® Q5® Hot Start HiFi PCR Master Mix 50 reactions 1
M0480S OneTaq® DNA Polymerase 200 units 2
M0481S OneTaq® Hot Start DNA Polymerase 200 units 1
M0532S Phusion® High-Fidelity PCR Master Mix with GC Buffer 100 reactions (50 µl) 2
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 4
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 2
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl) 4
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions 2
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl) 4
M0491S Q5® High-Fidelity DNA Polymerase 100 units 3
M0491L Q5® High-Fidelity DNA Polymerase 500 units 3
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions 6
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units 3
M0297S RNase H 250 units 2
M0314S RNase Inhibitor, Murine 3,000 units 3
M0203L T4 DNA Polymerase 750 units 3
M0203S T4 DNA Polymerase 150 units 2
M0270L Taq 2X Master Mix 500 reactions (50 µl) 3
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units 2
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units 2
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units 2
NEB # Protein Tools Size Quantity Available
P0704S PNGase F 15,000 units 1
P8107S Proteinase K, Molecular Biology Grade 2 ml 2
NEB # Restriction Endonucleases Size Quantity Available
R3552S AgeI-HF® 300 units 3
R0174S AvrII 100 units 2
R3136S BamHI-HF® 10,000 units 3
R3539S BbsI-HF® 300 units 2
R0144S BglII 2,000 units 1
R3733S BsaI-HF®v2 1,000 units 2
R0739S BsmBI-v2 200 units 2
R0197S ClaI 1,000 units 2
R3101S EcoRI-HF® 10,000 units 3
R3195S EcoRV-HF® 4,000 units 2
R3104S HindIII-HF® 10,000 units 3
R3142S KpnI-HF® 4,000 units 3
R3193S NcoI-HF® 1,000 units 3
R0111S NdeI 4,000 units 2
R3131L NheI-HF® 5,000 units 2
R3131S NheI-HF® 1,000 units 3
R3189S NotI-HF® 500 units 2
R3189L NotI-HF® 2,500 units 2
R0547S PacI 250 units 3
R3122S ScaI-HF® 1,000 units 2
R0141S SmaI 2,000 units 3
R3133S SpeI-HF® 500 units 2
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 1
R0146S XhoI 5,000 units 3
R0180S XmaI 500 units 3
NEB # RNA Reagents Size Quantity Available
M0303S DNase I (RNase-free) 1,000 units 3
E2040S HiScribe™ T7 High Yield RNA Synthesis Kit 50 reactions 2
M0525S Quick CIP 1,000 units 2

NEBnow_FreezerPage_FAQs_Icon  

NEBnow FAQs


Find the answers to some common questions below. Still have a question? Please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).

Registration

How do I gain access to the NEBnow Freezer?

You can request access by completing an NEBnow registration form. Click Register for Access under My NEB Freezer Location and Hours.

When will I receive access to the NEBnow Freezer?

NEB will receive your request and will verify your information with your Institutional Freezer Coordinator. Once authorized, you will receive an email with your access information. You should receive this email within 2 business days of submitting your request.

How do I change my user registration information?

To change or update your contact information, please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com)


Scanning

What if my product cannot be scanned?

If you have difficulty scanning your product’s barcode, you can select the Manual Entry button, which can be found on any of the product screens. Type in the product code, and the product will be added to your running order.

Do I have to scan each individual product that I remove from the freezer?

Yes, each product removed or returned to the freezer must be scanned. Each product you scan will be added to or removed from your order. You will have the opportunity to review and confirm your order prior to checkout.

What is a special order?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.


Freezer restocking

How often are the NEBnow freezer products replenished?

NEBnow freezer inventory is replenished on a regular basis. Find your freezer's restocking day by viewing Products Available in this Freezer.

When restocking the freezer, what if the shipping/picking list barcode does not scan?

If the picking list barcode does not scan, please contact NEBnow Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).


Product

How do I locate the product I want in the freezer?

Refer to the map located on the freezer door to locate the tray in which your product is located.

What is a “Special Order"?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated and delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.

How do I know what products are available fore special order?

All NEB catalog products are available for Special Order. Select the Special Order button on the home screen to browse NEB products.

How do I place a special order from the website?

At this time, all special orders need to be initiated at participating freezers. See Learn more about your NEBnow freezer for instruction on how to place a special order.


Password

What if I forget my NEBnow PIN?

After three failed attempts of logging into the NEBnow Freezer, you will receive an email providing you with a new PIN. Alternatively, you may contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com) for support.


Other

What should I do if the freezer appears not to be cold enough or not holding its temperature?

Please contact your local Institutional Freezer Coordinator if you notice problems with the freezer.