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Harvard Medical School BPF NEBnow


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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
B7021S Gel Loading Dye, Blue (6X) 4 ml B7021S
T1031L Monarch® DNA Cleanup Binding Buffer 235 ml T1031L
T1021L Monarch® Gel Dissolving Buffer 235 ml T1021L
B6058S NEBNext® Quick Ligation Reaction Buffer 2 ml B6058S
B0202S T4 DNA Ligase Reaction Buffer 6 ml B0202S
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Stockroom #
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions E0552S
NEB # DNA Modifying Enzymes Size Stockroom #
M0293S Exonuclease I (E. coli) 3,000 units M0293S
M0293L Exonuclease I (E. coli) 15,000 units M0293L
E2611L Gibson Assembly® Master Mix 50 reactions E2611L
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
M0370S Instant Sticky-end Ligase Master Mix 50 reactions M0370S
M0212S Klenow Fragment (3'→5' exo-) 200 units M0212S
M0212L Klenow Fragment (3'→5' exo-) 1,000 units M0212L
M0247S Micrococcal Nuclease 320,000 gel units M0247S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions E2621L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M2200L Quick Ligation™ Kit 150 reactions M2200L
M0202M T4 DNA Ligase 100,000 units M0202M
M0202T T4 DNA Ligase 20,000 units M0202T
M0202L T4 DNA Ligase 100,000 units M0202L
M0202S T4 DNA Ligase 20,000 units M0202S
M0208S Taq DNA Ligase 2,000 units M0208S
M0208L Taq DNA Ligase 10,000 units M0208L
M5505L USER® Enzyme 250 units M5505L
NEB # DNA Plasmids & Substrates Size Stockroom #
N4040S M13mp18 Single-stranded DNA 10 µg N4040S
NEB # Epigenetics Size Stockroom #
R0176L DpnI 5,000 units R0176L
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Glycobiology Size Stockroom #
P0702S Endo H 10,000 units P0702S
P0704S PNGase F 15,000 units P0704S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3232L 1 kb DNA Ladder 1 ml N3232L
N3200L 1 kb Plus DNA Ladder 0.5 ml N3200L
N3200S 1 kb Plus DNA Ladder 0.1 ml N3200S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N0468S Quick-Load® 1 kb DNA Ladder 1.25 ml N0468S
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml N0467S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
N3014S λ DNA-BstEII Digest 0.3 ml N3014S
NEB # Nucleic Acid Purification Size Stockroom #
T1034L Monarch® DNA Cleanup Columns (5 μg) 100 columns T1034L
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T1020L Monarch® DNA Gel Extraction Kit 250 preps T1020L
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps T1030S
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps T1030L
T1010L Monarch® Plasmid Miniprep Kit  250 preps T1010L
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
P0756S Adenosine 5'-Triphosphate (ATP) 1 ml P0756S
M0537S Bst 2.0 DNA Polymerase 1,600 units M0537S
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions E3005L
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
E3006L Luna® Universal Probe One-Step RT-qPCR Kit 500 reactions E3006L
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions E3006S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3004L Luna® Universal Probe qPCR Master Mix 500 rxn (5 x 1 ml) M3004L
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M3003L Luna® Universal qPCR Master Mix 500 rxn (5 x 1 ml) M3003L
E3010S LunaScript™ RT SuperMix Kit 25 reactions E3010S
M0543S NEBNext® Q5® Hot Start HiFi PCR Master Mix 50 reactions M0543S
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0481S OneTaq® Hot Start DNA Polymerase 200 units M0481S
M0532S Phusion® High-Fidelity PCR Master Mix with GC Buffer 100 reactions (50 µl vol) M0532S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) M0531S
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl vol) M0492S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) M0494S
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0203S T4 DNA Polymerase 150 units M0203S
M0203L T4 DNA Polymerase 750 units M0203L
M0270L Taq 2X Master Mix 500 reactions (50 µl vol) M0270L
M0320L Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 2,000 units M0320L
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0174S AvrII 100 units R0174S
R3136S BamHI-HF® 10,000 units R3136S
R3539S BbsI-HF® 300 units R3539S
R0144S BglII 2,000 units R0144S
R0580S BsmBI 200 units R0580S
R0162S BstEII 2,000 units R0162S
R0197S ClaI 1,000 units R0197S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3131L NheI-HF® 5,000 units R3131L
R0189M NotI 2,500 units R0189M
R3189S NotI-HF® 500 units R3189S
R3189L NotI-HF® 2,500 units R3189L
R0547S PacI 250 units R0547S
R3122S ScaI-HF® 1,000 units R3122S
R0141S SmaI 2,000 units R0141S
R3133S SpeI-HF® 500 units R3133S
R3182S SphI-HF® 500 units R3182S
R3132S SspI-HF® 1,000 units R3132S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
T2010S Monarch® Total RNA Miniprep Kit 50 preps T2010S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol of each N0447L
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
E2040S HiScribe™ T7 High Yield RNA Synthesis Kit 50 reactions E2040S
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps T2030S
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions E6560S
M0368S ProtoScript® II Reverse Transcriptase 4,000 units M0368S
M0297S RNase H 250 units M0297S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S
M0201L T4 Polynucleotide Kinase 2,500 units M0201L
M0204S T4 RNA Ligase 1 (ssRNA Ligase) 1,000 units M0204S
NEB # Sample Prep for NGS & Target Enrichment Size Stockroom #
E7335S NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) 24 reactions E7335S

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