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Harvard Medical School BPF NEBnow


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NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 2
B7204S CutSmart® Buffer 5 ml 2
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
B7021S Gel Loading Dye, Blue (6X) 4 ml 2
T1031L Monarch® DNA Cleanup Binding Buffer 235 ml 1
T1021L Monarch® Gel Dissolving Buffer 235 ml 1
B0202S T4 DNA Ligase Reaction Buffer 6 ml 2
B9004S ThermoPol® Reaction Buffer Pack 6 ml (4 x 1.5 ml) 2
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Quantity Available
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions 2
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
M0293L Exonuclease I (E. coli) 15,000 units 2
M0293S Exonuclease I (E. coli) 3,000 units 2
E2611S Gibson Assembly® Master Mix 10 reactions 2
E2611L Gibson Assembly® Master Mix 50 reactions 2
M0370S Instant Sticky-end Ligase Master Mix 50 reactions 2
M0212L Klenow Fragment (3'→5' exo-) 1,000 units 2
M0212S Klenow Fragment (3'→5' exo-) 200 units 2
M0247S Micrococcal Nuclease 320,000 gel units 3
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 2
M2200S Quick Ligation™ Kit 30 reactions 2
M2200L Quick Ligation™ Kit 150 reactions 2
M0202T T4 DNA Ligase 20,000 units 2
M0202L T4 DNA Ligase 100,000 units 2
M0202S T4 DNA Ligase 20,000 units 2
M0202M T4 DNA Ligase 100,000 units 2
M0208S Taq DNA Ligase 2,000 units 2
M0208L Taq DNA Ligase 10,000 units 2
M5505L USER® Enzyme 250 units 1
NEB # Epigenetics Size Quantity Available
R0176L DpnI 5,000 units 2
R0176S DpnI 1,000 units 2
R3138S SalI-HF® 2,000 units 2
NEB # Glycobiology Size Quantity Available
P0702S Endo H 10,000 units 1
P0704S PNGase F 15,000 units 1
NEB # Markers & Ladders Size Quantity Available
N3232S 1 kb DNA Ladder 0.2 ml 2
N3232L 1 kb DNA Ladder 1 ml 2
N3200L 1 kb Plus DNA Ladder 0.5 ml 2
N3200S 1 kb Plus DNA Ladder 0.1 ml 2
N3231L 100 bp DNA Ladder 0.5 ml 2
N3231S 100 bp DNA Ladder 0.1 ml 2
N0468S Quick-Load® 1 kb DNA Ladder 1.25 ml 2
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml 2
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml 1
NEB # Nucleic Acid Purification Size Quantity Available
T1034L Monarch® DNA Cleanup Columns (5 μg) 100 columns 1
T1020S Monarch® DNA Gel Extraction Kit 50 preps 3
T1020L Monarch® DNA Gel Extraction Kit 250 preps 2
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps 2
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 2
T1010L Monarch® Plasmid Miniprep Kit  250 preps 2
T1010S Monarch® Plasmid Miniprep Kit  50 preps 2
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
P0756S Adenosine 5'-Triphosphate (ATP) 1 ml 2
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions 1
E3006L Luna® Universal Probe One-Step RT-qPCR Kit 500 reactions 2
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions 2
M3004L Luna® Universal Probe qPCR Master Mix 500 rxn (5 x 1 ml) 2
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) 2
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
M3003L Luna® Universal qPCR Master Mix 500 rxn (5 x 1 ml) 2
E3010S LunaScript™ RT SuperMix Kit 25 reactions 2
M0543S NEBNext® Q5® Hot Start HiFi PCR Master Mix 50 reactions 1
M0480S OneTaq® DNA Polymerase 200 units 2
M0481S OneTaq® Hot Start DNA Polymerase 200 units 2
M0532S Phusion® High-Fidelity PCR Master Mix with GC Buffer 100 reactions (50 µl vol) 2
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 2
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 2
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) 2
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl vol) 4
M0491L Q5® High-Fidelity DNA Polymerase 500 units 2
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) 2
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units 2
M0203S T4 DNA Polymerase 150 units 2
M0203L T4 DNA Polymerase 750 units 2
M0270L Taq 2X Master Mix 500 reactions (50 µl vol) 2
M0320L Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 2,000 units 2
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units 2
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units 2
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units 2
NEB # Protein Tools Size Quantity Available
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials 2
NEB # Restriction Endonucleases Size Quantity Available
R3552S AgeI-HF® 300 units 1
R0174S AvrII 100 units 2
R3136S BamHI-HF® 10,000 units 2
R3539S BbsI-HF® 300 units 2
R0144S BglII 2,000 units 2
R3733S BsaI-HF®v2 1,000 units 2
R0739S BsmBI-v2 200 units 2
R0197S ClaI 1,000 units 2
R3101S EcoRI-HF® 10,000 units 2
R3195S EcoRV-HF® 4,000 units 3
R3104S HindIII-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 1
R3193S NcoI-HF® 1,000 units 2
R0111S NdeI 4,000 units 2
R3131L NheI-HF® 5,000 units 2
R3131S NheI-HF® 1,000 units 1
R0189M NotI 2,500 units 1
R3189S NotI-HF® 500 units 2
R3189L NotI-HF® 2,500 units 2
R0547S PacI 250 units 2
R3122S ScaI-HF® 1,000 units 2
R0141S SmaI 2,000 units 2
R3133S SpeI-HF® 500 units 1
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 2
R0146S XhoI 5,000 units 2
R0180S XmaI 500 units 2
NEB # RNA Reagents Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 3
M0289S Antarctic Phosphatase 1,000 units 2
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol of each 2
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units out of stock
E2040S HiScribe™ T7 High Yield RNA Synthesis Kit 50 reactions 2
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps out of stock
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps 2
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps 2
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions 2
M0368S ProtoScript® II Reverse Transcriptase 4,000 units 2
M0525S Quick CIP 1,000 units 2
M0297S RNase H 250 units 2
M0314S RNase Inhibitor, Murine 3,000 units out of stock
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 2
M0201S T4 Polynucleotide Kinase 500 units 2
M0201L T4 Polynucleotide Kinase 2,500 units 2

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