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Harvard Medical School BPF NEBnow

My NEB Freezer Location and Hours


Location:
Biopolymers Facilties Self Service Supply Center
142 Goldenson Building, 200 Longwood Avenue
Boston, MA 02115

Hours:
NEBnow® Kiosk, 24 x 7 access

Contact:
James Caffery
Phone: (617) 432-7480
Fax:(617) 432-7440
freezer@genome.med.harvard.edu

Register for Access
NEBNow Purchasing From Freezer
NEBnow® Purchasing from Your NEB Product Freezer

Learn how to purchase products from an NEBnow freezer. It’s as quick and easy as 1, 2, 3…

Watch Tutorial
joanna_hamilton
My NEB Academic Accounts

Joanna Hamilton, Ph.D.
Phone: 978-471-2382
jhamilton@neb.com

Need technical support?
Contact our technical support team to help with your experiments and answer your questions.


Learn more about your NEBnow freezer

NEBnow_FreezerPage_Products_Icon  

Products Available in this Freezer


Inventory is replenished every Wednesday. See below for the regularly stocked product list and their estimated availability as of 9/24/2023 4:30 AM.

Are you interested having in an NEB product added to the regularly stocked inventory? Please tell us which product(s) and we'll contact your NEB Account Manager about your request.

Add a Product to Inventory
NEB # Buffers Size
B7024S Gel Loading Dye, Purple (6X) 4 ml
B7021S Gel Loading Dye, Blue (6X) 4 ml
T1031L Monarch® DNA Cleanup Binding Buffer 175 ml
T1021L Monarch® Gel Dissolving Buffer 235 ml
B6058S NEBNext® Quick Ligation Reaction Buffer 2 ml
B7002S NEBuffer™ 2 5 ml
B9004S ThermoPol® Reaction Buffer 6 ml
NEB # DNA Assembly Cloning and Mutagenesis Kits Size
E2611L Gibson Assembly® Master Mix 50 reactions
E2611S Gibson Assembly® Master Mix 10 reactions
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions
NEB # DNA Modifying Enzymes and Cloning Technologies Size
M0289S Antarctic Phosphatase 1,000 units
M0298S Cre Recombinase 50 units
M0293S Exonuclease I (E. coli) 3,000 units
M0293L Exonuclease I (E. coli) 15,000 units
M0370S Instant Sticky-end Ligase Master Mix 50 reactions
M0212S Klenow Fragment (3'→5' exo-) 200 units
M0212L Klenow Fragment (3'→5' exo-) 1,000 units
M0262S Lambda Exonuclease 1,000 units
M0262L Lambda Exonuclease 5,000 units
M0247S Micrococcal Nuclease 320,000 gel units
M2200L Quick Ligation™ Kit 150 reactions
M2200S Quick Ligation™ Kit 30 reactions
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units
M0202S T4 DNA Ligase 20,000 units
M0202L T4 DNA Ligase 100,000 units
M0202T T4 DNA Ligase 20,000 units
M0202M T4 DNA Ligase 100,000 units
B0202S T4 DNA Ligase Reaction Buffer 5 ml
M0201L T4 Polynucleotide Kinase 2,500 units
M0201S T4 Polynucleotide Kinase 500 units
M0208L Taq DNA Ligase 10,000 units
M0208S Taq DNA Ligase 2,000 units
M0281S Uracil Glycosylase Inhibitor (UGI) 200 units
M5505L USER® Enzyme 250 units
N4040S M13mp18 Single-stranded DNA 10 µg
NEB # Epigenetics Size
R0176L DpnI 5,000 units
R0176S DpnI 1,000 units
R3138S SalI-HF® 2,000 units
M0386S Cas9 Nuclease, S. pyogenes 90 pmol
NEB # Markers & Ladders Size
N3232S 1 kb DNA Ladder 200 gel lanes (0.2 ml)
N3232L 1 kb DNA Ladder 1,000 gel lanes (1 ml)
N3200S 1 kb Plus DNA Ladder 200 gel lanes (0.1 ml)
N3200L 1 kb Plus DNA Ladder 1,000 gel lanes (0.5 ml)
N3231L 100 bp DNA Ladder 500 gel lanes (0.5 ml)
N3231S 100 bp DNA Ladder 100 gel lanes (0.1 ml)
N0468S Quick-Load® 1 kb DNA Ladder 125 gel lanes (1.25 ml)
N0467S Quick-Load® 100 bp DNA Ladder 125 gel lanes (1.25 ml)
N0552S Quick-Load® Purple 1 kb DNA Ladder 125 gel lanes (1.25 ml)
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 250 gel lanes (1.25 ml)
N0551S Quick-Load® Purple 100 bp DNA Ladder 125 gel lanes (1.25 ml)
N3014S λ DNA-BstEII Digest 150 gel lanes (0.3 ml)
E7335S NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) 24 reactions (12 indices)
NEB # Nucleic Acid Purification Size
T2010S Monarch® Total RNA Miniprep Kit 50 preps
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps
T1034L Monarch® DNA Cleanup Columns (5 μg) 100 columns
T1020L Monarch® DNA Gel Extraction Kit 250 preps
T1020S Monarch® DNA Gel Extraction Kit 50 preps
T3010L Monarch® Genomic DNA Purification Kit  150 preps
T3010S Monarch® Genomic DNA Purification Kit  50 preps
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps
T1010S Monarch® Plasmid Miniprep Kit  50 preps
T1010L Monarch® Plasmid Miniprep Kit  250 preps
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps
NEB # PCR, Polymerases & Amplification Technologies Size
P0756S Adenosine 5'-Triphosphate (ATP) 1 ml
M0537S Bst 2.0 DNA Polymerase 1,600 units
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions
E3006L Luna® Universal Probe One-Step RT-qPCR Kit 500 reactions
M3004L Luna® Universal Probe qPCR Master Mix 500 reactions
M3004S Luna® Universal Probe qPCR Master Mix 200 reactions
M3003S Luna® Universal qPCR Master Mix 200 reactions
M3003L Luna® Universal qPCR Master Mix 500 reactions
E3010S LunaScript® RT SuperMix Kit 25 reactions
M0543S NEBNext® Q5® Hot Start HiFi PCR Master Mix 50 reactions
M0480S OneTaq® DNA Polymerase 200 units
M0481S OneTaq® Hot Start DNA Polymerase 200 units
M0532S Phusion® High-Fidelity PCR Master Mix with GC Buffer 100 reactions (50 µl)
M0530L Phusion® High-Fidelity DNA Polymerase 500 units
M0530S Phusion® High-Fidelity DNA Polymerase 100 units
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl)
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions
M0368S ProtoScript® II Reverse Transcriptase 4,000 units
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl)
M0491S Q5® High-Fidelity DNA Polymerase 100 units
M0491L Q5® High-Fidelity DNA Polymerase 500 units
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units
M0297S RNase H 250 units
M0314S RNase Inhibitor, Murine 3,000 units
M0203L T4 DNA Polymerase 750 units
M0203S T4 DNA Polymerase 150 units
M0270L Taq 2X Master Mix 500 reactions (50 µl)
M0320L Taq DNA Polymerase with Standard Taq (Mg-free) Buffer 2,000 units
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units
NEB # Protein Tools Size
P0702S Endo H 10,000 units
P0704S PNGase F 15,000 units
P8107S Proteinase K, Molecular Biology Grade 2 ml
NEB # Restriction Endonucleases Size
R3552S AgeI-HF® 300 units
R0558S AscI 500 units
R0174S AvrII 100 units
R3136S BamHI-HF® 10,000 units
R3539S BbsI-HF® 300 units
R0144S BglII 2,000 units
R3733S BsaI-HF®v2 1,000 units
R0739S BsmBI-v2 200 units
R0197S ClaI 1,000 units
R3101S EcoRI-HF® 10,000 units
R3195S EcoRV-HF® 4,000 units
R3104S HindIII-HF® 10,000 units
R0105S HpaI 500 units
R3142S KpnI-HF® 4,000 units
R0648L Nb.BsrDI 5,000 units
R3193S NcoI-HF® 1,000 units
R0111S NdeI 4,000 units
R3131S NheI-HF® 1,000 units
R3131L NheI-HF® 5,000 units
R0189M NotI 2,500 units
R3189L NotI-HF® 2,500 units
R3189S NotI-HF® 500 units
R0547L PacI 1,250 units
R0547S PacI 250 units
R0560S PmeI 500 units
R3156S SacI-HF® 2,000 units
R3122S ScaI-HF® 1,000 units
R0141S SmaI 2,000 units
R3133S SpeI-HF® 500 units
R3182S SphI-HF® 500 units
R3132S SspI-HF® 1,000 units
R0145S XbaI 3,000 units
R0146S XhoI 5,000 units
R0180S XmaI 500 units
NEB # RNA Reagents Size
M0303S DNase I (RNase-free) 1,000 units
E2040S HiScribe® T7 High Yield RNA Synthesis Kit 50 reactions
M0525S Quick CIP 1,000 units
M0204S T4 RNA Ligase 1 (ssRNA Ligase) 1,000 units
NEBnow_FreezerPage_FAQs_Icon  

NEBnow FAQs


Find the answers to some common questions below. Still have a question? Please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).

Registration

How do I gain access to the NEBnow Freezer?

When will I receive access to the NEBnow Freezer?

How do I change my user registration information?

Scanning

What if my product cannot be scanned?

Do I have to scan each individual product that I remove from the freezer?

What is a "Special Order"?

Freezer Restocking

How often are the NEBnow freezer products replenished?

When restocking the freezer, what if the shipping/picking list barcode does not scan?

Product

How do I locate the product I want in the freezer?

What is a "Special Order"?

How do I know what products are available for special order?

How do I place a special order from the website?

Password

What if I forget my NEBnow PIN?

Other

What should I do if the freezer appears not to be cold enough or not holding its temperature?

Registration

How do I gain access to the NEBnow Freezer?

You can request access by completing an NEBnow registration form. Click Register for Access under My NEB Freezer Location and Hours.

When will I receive access to the NEBnow Freezer?

NEB will receive your request and will verify your information with your Institutional Freezer Coordinator. Once authorized, you will receive an email with your access information. You should receive this email within 2 business days of submitting your request.

How do I change my user registration information?

To change or update your contact information, please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).


Scanning

What if my product cannot be scanned?

If you have difficulty scanning your product’s barcode, you can select the Manual Entry button, which can be found on any of the product screens. Type in the product code, and the product will be added to your running order.

Do I have to scan each individual product that I remove from the freezer?

Yes, each product removed or returned to the freezer must be scanned. Each product you scan will be added to or removed from your order. You will have the opportunity to review and confirm your order prior to checkout.

What is a special order?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.


Freezer restocking

How often are the NEBnow freezer products replenished?

NEBnow freezer inventory is replenished on a regular basis. Find your freezer's restocking day by viewing Products Available in this Freezer.

When restocking the freezer, what if the shipping/picking list barcode does not scan?

If the picking list barcode does not scan, please contact NEBnow Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).


Product

How do I locate the product I want in the freezer?

Refer to the map located on the freezer door to locate the tray in which your product is located.

What is a “Special Order"?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated and delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.

How do I know what products are available for special order?

All NEB catalog products are available for Special Order. Select the Special Order button on the home screen to browse NEB products.

How do I place a special order from the website?

At this time, all special orders need to be initiated at participating freezers. See Learn more about your NEBnow freezer for instruction on how to place a special order.


Password

What if I forget my NEBnow PIN?

After three failed attempts of logging into the NEBnow Freezer, you will receive an email providing you with a new PIN. Alternatively, you may contact NEB Customer Service by telephone (1-800-632-5227) or (freezers@neb.com).


Other

What should I do if the freezer appears not to be cold enough or not holding its temperature?

Please contact your local Institutional Freezer Coordinator if you notice problems with the freezer.

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.

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