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DNA Repair Enzymes and Structure-specific Endonucleases

DNA Repair Enzymes and Structure-specific Endonucleases are enzymes which cleave DNA at a specific DNA lesion or structure. (To learn about non-specific endonucleases and exonucleases, visit here.)  These enzymes can be used in a wide variety of applications such as: 

  • Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
  • Base excision repair (BER)
  • DNA mismatch repair
  • Nucleotide excision repair
  • Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
  • Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
  • Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
  • Genotoxicity tests by alkaline elution or alkaline unwinding
  •  Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)

Helping you select the right DNA Repair Enzymes and Structure-specific Endonucleases

NEB offers a comprehensive selection of DNA repair enzymes and structure-specific endonucleases, all of which have been optimized for robust performance in streamlined workflows.

Additional tools and resources

The following resources will help you select and learn more about DNA Repair Enzymes and Structure-specific Endonucleases from NEB.

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FAQs for DNA Repair Enzymes and Structure-specific Endonucleases
Protocols for DNA Repair Enzymes and Structure-specific Endonucleases
    Publications related to DNA Repair Enzymes and Structure-specific Endonucleases
    • Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. (2015) Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol Res; 4, 725-738. DOI: doiL 10.1039/c4tx00197d
    • Mauris, J.and Evans, T.C., Jr. (2010) A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem; 285(15), 11087-11092. PubMedID: 20129926
    • Gehring, A.M., Zatopek, K.M., Burkhart, B.W., Potapov, V., Santangelo, T.J., Gardner, A.F (2019) Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis DNA Repair (Amst); PubMedID: 31841800, DOI: 10.1016/j.dnarep.2019.102767
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