Home FAQs How does Authenticase® improve the quality and fidelity of PCR gene assembly?

FAQ: How does Authenticase® improve the quality and fidelity of PCR gene assembly?

Authenticase is a proprietary mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel (insertion and/or deletion) regions, ranging from 1-10 base pairs (bp) on double-stranded DNA. By enzymatically removing “mistakes” prior to the final renaturation and amplification step, Authenticase can reduce errors in oligo-based PCR gene assembly.