FAQ: Can I use Authenticase™ for genome editing applications?

Yes. Authenticase can be used to detect mismatch/indel formation (on-target editing events) in genome editing workflows. It has been formulated to efficiently cleave mismatches in heteroduplex DNA and outperforms traditional T7 Endo I screening across a broad range of mutation/WT ratios. Please see Mismatch Detection Assay (NEB #M0689).