FAQ: Why do you recommend setting up two tubes for the PCR reaction containing different amounts of Authenticase®-treated samples as templates?

DIY gene synthesis workflows often enrich the full-length target gene prior to DNA assembly or cloning into the holding vector. We do not require the user to set up multiple enrichment reactions; however, since amplification by PCR can produce variable amounts of the full-length target, we suggest establishing more than one PCR reaction to ensure a sufficient amount of the full-length target is generated. After PCR, select the amplicon with the higher purity by running an agarose gel analysis or Bioanalyzer prior to cloning the fragment.