FAQ: Does Authenticase™ recognize single base pair mismatches or indels (insertions/deletions)?

The answer is complex. There are eight types of single base mismatches: C/C, T/C, A/C, T/G, A/G, G/G, T/T and A/A. Authenticase can recognize 7 of the 8 mismatches. The exception is an A/G mismatch. Cleavage of these mismatches using Authenticase is more efficient than with T7 Endonuclease I, which has limited activity on these single-base mismatches (Biochemistry 2004, 43, 4313-4322). Both enzymes have no problem cleaving heteroduplex DNA with 1-10 bp Indels or 2-10 bp mismatches.