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Exonucleases and Non-specific Endonucleases

Exonucleases catalyze the removal of nucleotides in either the 5´ to 3´ or the 3´ to 5´ direction from single- or double-stranded DNA. These enzymes can be used in a wide variety of applications, including next-generation sequencing (NGS), PCR and gene synthesis.

Non-specific endonucleases cleave DNA and are generally used for DNA contamination removal. (To learn about DNA Repair Enzymes and Structure-specific endonucleases, visit here.)

Helping you select the right exonucleases and non-specific endonucleases

NEB offers a comprehensive selection of exonucleases and non-specific endonucleases for the cleavage of DNA, all of which have been optimized for robust performance in streamlined workflows.
Choosing the Right Exonuclease
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Additional tools and resources

The following resources will help you select and learn more about exonucleases and non-specific endonucleases from NEB.

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Protocols for Exonucleases and Non-specific Endonucleases
Application Notes for Exonucleases and Non-specific Endonucleases
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