Home Discontinued EagI

EagI

This product has been discontinued and is replaced with the High-Fidelity version, EagI-HF (NEB #R3505)

Catalog # R0505 was discontinued on March 17, 2021

NEB restriction endonuclease that recognizes the sequence C^GGCC_G

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #3505) supplied with CutSmart^® Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: C/GGCCG

Product Information
EagI has a High Fidelity version, EagI-HF® (NEB #R3505)

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source
An E. coli strain that carries the EagI gene from Enterobacter agglomerans (R. Morgan).

This product is related to the following categories:

Discontinued (<3 years)
- Properties & Usage
  Unit Definition
  One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
  Reaction Conditions
  
  1X NEBuffer™ 3.1
  Incubate at 37°C
  
  1X NEBuffer™ 3.1
  100 mM NaCl
  50 mM Tris-HCl
  10 mM MgCl₂
  100 µg/ml BSA
  (pH 7.9 @ 25°C)
  
  Activity in NEBuffers
  NEBuffer™ 1.1: 10%
  NEBuffer™ 2.1: 25%
  NEBuffer™ 3.1: 100%
  
  Diluent Compatibility
  
  Diluent B
  
  Storage Buffer
  
  10 mM Tris-HCl
  500 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  200 µg/ml BSA
  50% Glycerol
  pH 8 @ 25°C
  
  Heat Inactivation
  65°C for 20 minutes
  Methylation Sensitivity
  
  dam methylation: Not Sensitive
  dcm methylation: Not Sensitive
  CpG Methylation: Blocked
- Related Products
  Companion Products
  
  EagI-HF®
  
  Materials Sold Separately
  
  Gel Loading Dye, Purple (6X)
  
  NEBuffer 3.1
- Product Notes
  EagI is an isoschizomer of XmaIII.
  
  For full EagI activity, the pH of the reaction mix must be between (7.9 and 9.0 @ 25°C).
  
  This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
  
  Blocked by CpG methylation.
Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Digest Protocol
  
  Double Digest Protocol with Standard Restriction Enzymes
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Dam and Dcm Methylases of E. coli
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Effects of CpG Methylation on Restriction Enzyme Cleavage
  
  Heat Inactivation
  
  NEBuffer Activity/Performance Chart with Restriction Enzymes
  
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Star Activity
  
  Traditional Cloning Quick Guide
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Sequences
  
  Cleavage of Supercoiled DNA
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoelectric Points (pI) for Restriction Enzymes
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Recleavable Filled-in 5' Overhangs
  
  Time-Saver™ Qualified Enzymes
  
  Why Choose Recombinant Enzymes?
- Web Tools
  Competitor Cross-Reference Tool
  
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter™ v3.0
  
  NEBioCalculator®
  
  REBASE®
FAQs & Troubleshooting
- FAQs
  Does EagI produce commonly used compatible ends?
  
  Are more units of EagI required to cut supercoiled DNA than lambda DNA?
  
  Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  
  Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  
  Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Troubleshooting
  Restriction Enzyme Troubleshooting Guide
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0505M_v1
  
  R0505S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0505S_L_v1_0511401
  
  R0505M_v1_0471303
  
  R0505M_v1_0491307
  
  R0505S_L_v1_0471303
  
  R0505S_L_v1_0491308
  
  R0505S_L_v1_0521410
  
  R0505S_L_v1_0511410
  
  R0505M_v1_0521411
  
  R0505S_L_v1_0521411
  
  R0505S_L_v1_0541505
  
  R0505S_L_v1_0521407
  
  R0505M_v1_0511402
  
  R0505M_v1_0531501
  
  R0505M_v1_0531505
  
  R0505S_L_v1_0551602
  
  R0505S_L_v1_0551605
  
  R0505M_v1_0551602
  
  R0505M_v1_0551608
  
  R0505S_L_v1_0571611
  
  R0505M_v1_0571709
  
  R0505S_L_v1_0571705
  
  R0505S_L_v1_0571712
  
  R0505M_v1_0571803
  
  R0505L_v1_10008867
  
  R0505S_v1_10010521
  
  R0505M_v1_10021733
  
  R0505L_v1_10035405
  
  R0505M_v1_10035407
  
  R0505S_v1_10035409
  
  R0505S_v1_10043635
  
  R0505L_v1_10043637
  
  R0505M_v1_10050279
  
  R0505L_v1_10065277
  
  R0505S_v1_10062746
  
  R0505M_v1_10069598
  
  R0505M_v1_10077743
  
  R0505L_v1_10084611
  
  R0505S_v1_10084612
  
  R0505L_v1_10091754
  
  R0505M_v1_10091766
  
  R0505M_v1_10092489
  
  R0505L_v1_10098734
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  EagI
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Trademarks

Other Products You May Be Interested In

Loading Spinner