Monarch^® RNA Purification Columns

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
NEB # Stored at (°C) T2007L 25 Component Name Component # Stored at (°C) Amount Concentration Monarch® RNA Purification Columns T2007-1 25 2 x 50 columns Not Applicable Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

Companion Products

• Luna^® Universal One-Step RT-qPCR Kit
• Luna^® Universal Probe One-Step RT-qPCR Kit
• ProtoScript® II First Strand cDNA Synthesis Kit
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext® Poly(A) mRNA Magnetic Isolation Module
• ProtoScript® II Reverse Transcriptase

Materials Sold Separately

• Monarch^® Total RNA Miniprep Kit
• Monarch^® DNA/RNA Protection Reagent
• Monarch^® gDNA Removal Columns
• Monarch^® Collection Tubes II
• Monarch^® RNA Lysis Buffer
• Monarch^® RNA Priming Buffer
• Monarch^® RNA Wash Buffer
• Monarch^® Total RNA Miniprep Enzyme Pack
• Nuclease-free Water

1. Quick Protocol for Total RNA Extraction (NEB #T2010)
2. Total RNA Purification from Samples in RNAlater® using the Monarch Total RNA Miniprep Kit (NEB #T2010)
3. Total RNA Purification from TRIzol®-extracted Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
4. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
5. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
6. In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
7. Fractionation of Small and Large RNA Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
8. RNA Reaction Cleanup using the Monarch Total RNA Miniprep Kit (NEB #T2010)
9. Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
10. RNA Extraction from Saliva, Buccal Swabs, and Nasopharyngeal Swabs using the Monarch Total RNA Miniprep Kit (NEB #T2010)
11. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
12. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
13. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
14. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

• General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
• Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
• Guidelines for Purification of RNA from Cultured Mammalian Cells
• Guidelines for RNA Purification from Bacteria
• Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
• Guidelines for RNA Purification from Plants
• Guidelines for RNA Purification from Tissues 
• Guidelines for RNA Purification from Whole Blood
• Guidelines for RNA Purification from Yeast
• Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

• A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

1. Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
2. Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
3. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
4. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
5. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
7. Can I use the Monarch Total RNA Miniprep Kit for RNA cleanup after enzymatic manipulations?
8. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?
9. Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal cheek swabs?
10. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from plasma or serum?
11. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
12. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from drosophila?
13. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from zebrafish embryos/larvae?
14. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?
15. Are the Monarch gDNA Removal Columns (NEB #T2017) and Monarch RNA Purification Columns (NEB #T2007) included with the Monarch Total RNA Miniprep Kit (NEB #T2010) compatible with the QIACube® System?

• Troubleshooting Guide for Total RNA Extraction & Purification

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2007L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2007L_v1_10043830
• T2007L_v1_10044092
• T2007L_v1_10053941
• T2007L_v1_10074651
• T2007L_v1_10079497
• T2007L_v2_10097581
• T2007L_v2_10141104
• T2007L_v2_10144554
• T2007L_v2_10166724
• T2007L_v2_10175990
• T2007L_v2_10210907
• T2007L_v2_10247117

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.