DNA Library Prep for Illumina
New England Biolabs now offers two suites of reagents for DNA library prep, each solving a different set of challenges:
The NEBNext® Ultra™ II solution for Illumina DNA library prep is a streamlined suite of reagents and workflows designed for the construction of high-quality libraries, using input amounts from pg to μg of DNA. Workflows are fast, user-friendly, and automatable. Available with or without an integrated enzymatic fragmentation step and optional PCR-free workflows, NEBNext offers flexible product formats maximal flexibility in your workflows. Designed for use in concert with NEBNext Multiplex Oligos for simple yet powerful NGS sample multiplexing.
The NEBNext UltraExpress® reagents and workflow for DNA library prep are even more streamlined, enabling library prep in under 2 hours. With speed comes a tighter input amount range (10 – 200 ng), but for samples that fall within that input range, NEBNext UltraExpress offers a single-protocol solution; that means one adaptor concentration and one number of PCR cycles for all samples! And just like NEBNext Ultra II, our NEBNext UltraExpress DNA library prep kits are compatible with our NEBNext Multiplex Oligos.
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NEBNext Ultra II DNA & FS DNA Library Prep |
NEBNext UltraExpress DNA Library Prep |
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FEATURES: |
Unprecedented performance, enabling lower inputs and fewer PCR cycles for the broadest range of input amounts and qualities; input-to-library time: 5 hours |
Streamlined and straightforward DNA library prep with a simple, single-condition protocol for all inputs within the kit’s input range; input-to-library time: < 2 hours |
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DNA Library Prep Kits: |
NEBNext Ultra II DNA Library Prep Kit |
NEBNext UltraExpress DNA Library Prep Kit |
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Pre-sheared Input Amount |
500 pg – 1 µg of sheared DNA |
10 ng – 200 ng of sheared DNA |
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Modules available? |
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N/A |
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FEATURES: |
Unprecedented performance, enabling lower inputs and fewer PCR cycles for the broadest range of input amounts and qualities; input-to-library time: 5 hours |
Faster, more-streamlined library prep with integrated enzymatic fragmentation, end repair, and dA-tailing with a single enzyme mix; input-to-library time: <2 hours |
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FS DNA Library Prep Kits: |
NEBNext Ultra II FS DNA Library Prep Kit |
NEBNext UltraExpress FS DNA Library Prep Kit Without Beads: NEB #E3340 |
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Intact Input Amount |
100 pg – 500 ng of intact DNA |
10 – 200 ng of intact DNA |
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Modules available? |
Ultra II FS DNA Module NEB #E7810) |
N/A |
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Compatible with PCR-free workflows? |
Yes. NEBNext Ultra II DNA PCR-free and FS DNA PCR-free library prep kits are available |
We recommend NEBNext Ultra II DNA PCR-free and FS DNA PCR-free for your applications that require PCR-free library prep. |
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Compatible with FFPE DNA? |
For FFPE DNA library prep, we strongly recommend the NEBNext UltraShear FFPE DNA Library Prep Kit. We do not recommend use of NEBNext FS DNA kits for FFPE DNA. |
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Compatible with methylome analysis? |
We strongly recommend NEBNext EM-seq™ (NEB #E7120) for methylome analysis, however NEBNext DNA kits can be used. We do not recommend use of NEBNext FS DNA kits with methylated DNA due to the potential for loss of methyl marks. |
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Compatible with oxford nanopore technologies? |
No. We recommend the NEBNext Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7180). |
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NEBNext DNA library prep reagents are also available at scale for your high-throughput and/or development needs. Contact the Customized Solutions Team for more details on custom product options, large volumes, or OEM materials.
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The Quantitation Question: How does accurate library quantitation influence sequencing?
The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.
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Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction
Among the available options for DNA fragmentation, enzymatic fragmentation is demonstrably gentler on the sample, yielding less damage at any scale. The new NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® includes a single step for enzymatic fragmentation, end repair, and dA tailing.
- A Robust, Streamlined, Enzyme-based DNA Library Preparation Method Amenable to a Wide Range of DNA Inputs (2019)
- EM-seq™ Enables Accurate and Precise Methylome Analysis of Challenging DNA Samples (2019)
- NEBNext® Ultra™ II FS DNA: A Robust Enzyme-based DNA Library Preparation Method Compatible with Plant Samples
- Uncovering the Cannabis sativa Methylome Through Enzymatic Methyl-seq (2019)
Feature Articles
Posters
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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NEBNEXT® Ultra II DNA Library Prep Protocol
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12 Quick Tips for NGS Library Preparation
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