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RNA Reagents

RNA plays a multitude of cellular roles in all kingdoms of life. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression.

In the last several years, our understanding of RNAs as regulatory molecules in the cell has dramatically changed. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology.

NEB offers a range of products for RNA research, including reagents for synthesis, extraction, purification, cleanup, analysis, modification and detection of RNA molecules.

Videos

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    In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. 

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    Tips for Successful RNA Purification using MONARCH® Total RNA Miniprep Kit

    Optimize the results of your RNA extractions with these simple tips from our scientists.

  3. MonarchTotal RNAMiniprep Protocol

    MONARCH® Total RNA Miniprep Protocol

    This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.

  4. Ong2018_BehindthePaper_720

    Base modifications affecting RNA polymerase and reverse transcriptase fidelity

    Behind the paper: Jennifer summarizes her group’s recent Nucleic Acid Research publication describing how individual RNA base modifications affect RNA polymerase fidelity as well as reverse transcription error rates.

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