RNA Reagents
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    • RNA Reagents

    RNA molecules play a multitude of cellular roles in all kingdoms of life. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression.

    In the last several years, our understanding of RNAs as regulatory molecules in the cell has dramatically changed. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology.

    Videos

    1. In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

      This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

    Learn More

    RNA Reagents includes these subcategories:

    miRNA & siRNA Information
    Reverse Transcriptases & RT-PCR Information
    RNA Isolation & Detection Information
    RNA Ligases Information
    RNA Markers & Ladders Information
    RNA Modification Information
    RNase Control Information
    RNases Information
    RNA Synthesis Information

      Publications related to RNA Reagents:

    1. Fuchs, R.T., Sun, Z., Zhuang, F., et al. (2015). Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049

    RNA Reagents Overview

    Reagents for RNA Sample Preparation

    NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.

    RNA Polymerase Selection Chart

    NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

    RNA Ligase Selection Chart

    NEB offers a variety of ligases for RNA research with unique specificities.

    cDNA Synthesis Selection Chart

    NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.