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RNA Reagents

RNA plays a multitude of cellular roles in all kingdoms of life. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression.

In the last several years, our understanding of RNAs as regulatory molecules in the cell has dramatically changed. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology.

NEB offers a range of products for RNA research, including reagents for synthesis, extraction, purification, cleanup, analysis, modification and detection of RNA molecules.

Choose Type:

RNA Reagents includes these subcategories:
miRNA & siRNA
Reverse Transcriptases & RT-PCR
RNA Extraction & Purification
RNA Isolation & Enrichment
RNA Ligases
RNA Markers & Ladders
RNA Modification
RNase Control
RNases
RNA Synthesis
FAQs for RNA Reagents
Protocols for RNA Reagents
    Publications related to RNA Reagents
  1. Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049
  2. Eyler, D.E., Franco, M.K., Batool, Z., Wu, M.Z., Dubuke, M.L., Dobosz-Bartoszek, M., Jones, J.D., Polikanov, Y.S., Roy, B., Koutmou, K.S 2019. Pseudouridinylatio of mRNA coding sequences alters translation Proc Natl Acad Sci U S A. 116(46), PubMedID: 31672910, DOI: 10.1073/pnas.1821754116
  3. Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S 2019. Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J Biol Chem. 294(48), PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
  4. Wu, M.Z., Asahara, H., Tzertzinis, G., Roy, B. 2020. Synthesis of low immunogenicity RNA with high-temperature in vitro transcription RNA. , PubMedID: 31900329, DOI:
  5. Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L 2018. Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Res. 46(11): 5753-5763, PubMedID: 29750267, DOI:
  6. Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; 2019. The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep. 9 (1), PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
RNA Polymerase Selection Chart
NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

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    In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. 

  2. NEBTV_tips_02_1920

    Tips for Successful RNA Purification using Monarch® Total RNA Miniprep Kit

    Optimize the results of your RNA extractions with these simple tips from our scientists.

  3. MonarchTotal RNAMiniprep Protocol

    Monarch® Total RNA Miniprep Protocol

    This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.

  4. Ong2018_BehindthePaper_720

    Base modifications affecting RNA polymerase and reverse transcriptase fidelity

    Behind the paper: Jennifer summarizes her group’s recent Nucleic Acid Research publication describing how individual RNA base modifications affect RNA polymerase fidelity as well as reverse transcription error rates.

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