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All High Fidelity (HF®) Restriction Enzymes are Time-Saver qualified and certified to be nuclease free, giving them the power to digest in 5-15 minutes and the purity to withstand overnight digestions. Choose the reaction time that works for you. For your convenience, NEB offers over 200 Time-Saver qualified enzymes.

EcoRI-HF™ (NEB #R3101) shows no star activity when used in 5 minutes or overnight. 50 μl reactions were set up using 1 μg of Lambda DNA, 1 μl of enzyme and recommended reaction buffer. Digests were incubated at 37°C. Marker M is the 1 kb DNA Ladder (NEB# N3232)

HF® is a registered trademark of New England Biolabs, Inc.
FastDigest® is a registered trademark of Fermentas.

Learn about High-Fidelity (HF®) Restriction Endonucleases