neb31 cloned at NEB recombinant time saver dil_C 25 65 Heat reduced star activity

CviAII was discontinued on September 15, 2023. FatI (NEB #R0650) or NlaIII (NEB #R0125) may be suitable replacements.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

  • Catalog # R0640 was discontinued on September 15, 2023
NEB restriction endonuclease that recognizes the sequence C^AT_G
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Restriction Enzyme Cut Site: C/ATG
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