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Home Restriction Endonucleases Products MscI

MscI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence TGG_^CCA

Isoschizomers | Single Letter Code | Pronunciation:

100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Restriction Enzyme Cut Site: TGG/CCA

Catalog #	Concentration	Size	List Price	Your Price
R0534S	5,000 units/ml	250 units	$77.00	Sign In Sign In
R0534L	5,000 units/ml	1,250 units	$317.00	Sign In Sign In
R0534M	25,000 units/ml	1,250 units	$317.00	Sign In Sign In

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Product Information

Product Source

An E. coli strain that carries the MscI gene from Micrococcus species (C. Polisson).

This product is related to the following categories:: Restriction Endonucleases H M

This product can be used in the following applications:: Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0534S -20

MscI R0534SVIAL -20 1 x 0.05 ml 5,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0534L -20

MscI R0534LVIAL -20 1 x 0.25 ml 5,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0534M -20

MscI R0534MVIAL -20 1 x 0.05 ml 25,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent C
Storage Buffer

10 mM Tris-HCl
150 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.05% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Blocked by Overlapping
CpG Methylation: Not Sensitive

Isoschizomers

BalI
MlsI
MluNI
Mox20I
Msp20I
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. MscI is an isoschizomer of BalI.
2. Inhibited by salt concentrations > 150 mM.
3. The single MscI site in pBR322 overlaps a dcm methylation site; consequently, pBR322 which has been grown in a dcm– host should be used for cloning.
4. Blocked by overlapping dcm methylation.