or

| |

neb.com will be undergoing maintenance on Sunday, June 11th, from 10am to 12pm US ET. If you experience an issue, please try again after the maintenance window. We apologize in advance for any inconvenience this may cause.

Home Discontinued Products SspI

SspI

This product was discontinued on 12/15/2022 and is replaced with the high fidelity version, SspI-HF™ (NEB #R3132).

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Replaced by SspI-HF® on December 15, 2022

Catalog # R0132 was discontinued on December 15, 2022

NEB restriction endonuclease that recognizes the sequence AAT_^ATT

Isoschizomers | Single Letter Code | Pronunciation:

High Fidelity (HF^®) version available (NEB #R3132) supplied with rCutSmart^™ Buffer
Restriction Enzyme Cut Site: AAT/ATT

Product Information
SspI has a High Fidelity version SspI-HF® (NEB #R3132).

High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the SspI gene from Sphaerotilus species (ATCC 13925).

This product is related to the following categories:

Discontinued (<3 years)
- Properties & Usage
  Unit Definition
  One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
  Reaction Conditions
  
  1X NEBuffer™ EcoRI/SspI
  Incubate at 37°C
  
  1X NEBuffer™ EcoRI/SspI
  100 mM Tris-HCl
  50 mM NaCl
  10 mM MgCl₂
  0.025% Triton® X-100
  (pH 7.5 @ 25°C)
  
  Activity in NEBuffers
  NEBuffer™ r1.1: 50%
  NEBuffer™ r2.1: 100%
  NEBuffer™ r3.1: 50%
  rCutSmart™ Buffer: 50%
  
  Diluent Compatibility
  
  Diluent C
  
  Storage Buffer
  
  10 mM Tris-HCl
  250 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  200 µg/ml BSA
  50% Glycerol
  0.15% Triton® X-100
  pH 7.4 @ 25°C
  
  Heat Inactivation
  65°C for 20 minutes
  Methylation Sensitivity
  
  dam methylation: Not Sensitive
  dcm methylation: Not Sensitive
  CpG Methylation: Not Sensitive
- Related Products
  Companion Products
  
  SspI-HF®
  
  Monarch® Plasmid Miniprep Kit
  
  Monarch® DNA Gel Extraction Kit
  
  Monarch® PCR & DNA Cleanup Kit (5 μg)
  
  Materials Sold Separately
  
  NEBuffer™ Set (EcoRI/SspI, DpnII)
- Product Notes
  Not sensitive to CpG, dcm, or dam methylation.
Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Digest Protocol
  
  Double Digest Protocol with Standard Restriction Enzymes
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Heat Inactivation
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Star Activity
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Sequences
  
  Cleavage of Supercoiled DNA
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoelectric Points (pI) for Restriction Enzymes
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Recleavable Blunt Ends
  
  Time-Saver™ Qualified Enzymes
- Web Tools
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter™ v3.0
  
  NEBioCalculator®
  
  REBASE®
FAQs & Troubleshooting
- FAQs
  What is Star Activity and how can it be avoided?
  
  Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  
  Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0132M_v1
  
  R0132S_L_v1
  
  R0132M_v2
  
  R0132S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0132M_v1_0351402
  
  R0132S_L_v1_0351402
  
  R0132M_v1_0341211
  
  R0132M_v1_0341303
  
  R0132M_v1_0341309
  
  R0132M_v1_0351408
  
  R0132S_L_v1_0341211
  
  R0132S_L_v1_0341303
  
  R0132S_L_v1_0341309
  
  R0132S_L_v1_0351408
  
  R0132M_v1_0351501
  
  R0132S_L_v1_0351501
  
  R0132M_v1_0351507
  
  R0132S_L_v1_0351507
  
  R0132M_v1_0351512
  
  R0132S_L_v1_0351511
  
  R0132S_L_v1_0351605
  
  R0132M_v1_0351608
  
  R0132M_v1_0351701
  
  R0132S_L_v1_0351701
  
  R0132S_L_v1_0351707
  
  R0132S_L_v1_0351710
  
  R0132M_v1_0351803
  
  R0132S_L_v1_0351804
  
  R0132L_v1_10008582
  
  R0132S_v1_10023545
  
  R0132M_v1_10030562
  
  R0132L_v1_10031405
  
  R0132M_v1_10043472
  
  R0132S_v1_10043469
  
  R0132L_v1_10043471
  
  R0132S_v1_10061347
  
  R0132L_v1_10061351
  
  R0132M_v1_10061350
  
  R0132S_v1_10088130
  
  R0132L_v1_10092896
  
  R0132M_v1_10092894
  
  R0132S_v1_10092941
  
  R0132S_v1_10110875
  
  R0132L_v1_10117152
  
  R0132M_v2_10110873
  
  R0132L_v2_10136004
  
  R0132S_v2_10136003
  
  R0132M_v2_10155975
  
  R0132S_v2_10155976
  
  R0132L_v2_10155977
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  SspI
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In

Loading Spinner