Total RNA Extraction & Purification

RNA plays a key role in gene expression and regulation. Extraction and purification of RNA from biological samples is an essential first step in the study of many biological processes. RNA extraction starts with homogenization of the sample followed by lysis of the cells to release the RNA. RNA is then conditioned to bind to a capture column, and washed to remove salts and other contaminants before it is eluted. Any contaminating genomic DNA can be removed with column-based or enzyme-based methods. 
The Monarch RNA Total Miniprep Kit is a universal kit for RNA extraction and purification from a wide variety of cell types including blood, cells, tissues, and tough-to-lyse samples (including bacterial and yeast). The kit includes a protection reagent for sample storage and lysis, Proteinase K for homogenization of some sample types, and gDNA removal columns and DNase I for gDNA removal. 
Commercial RNA cleanup kits are also available to remove reaction components, exchange buffers and/or concentrate samples. The Monarch Total RNA Purification Kit can be used to clean up RNA after enzymatic reactions before use in downstream workflows

FAQs for Total RNA Extraction & Purification

Protocols for Total RNA Extraction & Purification

Advantages of the Monarch Plasmid Miniprep Kit

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. NEBTV_tips_02_1920

    Tips for Successful RNA Purification using Monarch Total RNA Miniprep Kit

    Optimize the results of your RNA extractions with these simple tips from our scientists.

  2. MonarchTotal RNAMiniprep Protocol

    Monarch Total RNA Miniprep Protocol

    This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.

  3. RNase_Contamination_Anim_720x405

    Avoiding RNase Contamination

    Learn about the sources of RNase contamination and how you can prevent them from disrupting your experiments.

  4. Monarch_HowToRecycle_VideoThumb

    How to recycle your Monarch Kit components

    Learn how you can easily recycle all of the components in your Monarch Kits.