Quantitation of Isolated Poly(A) RNA from the Magnetic mRNA Isolation Kit (NEB #S1550)

At this point quantification of isolated poly(A)+ RNA can be done by measurement of the A260 absorbance using a Nanodrop™ spectrophotometer to avoid dilution of the sample. The A260/280 ratio should be 1.6 or greater.

The amount of isolated RNA will vary with the source of the RNA sample, typically for RNA 1 A260 Unit = ~40 μg/ml.

The eluted mRNA should be placed in the magnetic rack while removing aliquots for quantification to avoid pipetting of beads, which will interfere with spectrophotometric analysis.